Laccase-catalyzed synthesis and antioxidant property of poly(catechin)

被引：113

作者：

Kurisawa, M ^{[1
]}

Chung, JE

Uyama, H

Kobayashi, S

机构：

[1] Kyoto Univ, Grad Sch Engn, Dept Chem Mat, Kyoto 6158510, Japan

[2] Kyoto Univ, Biooriented Technol Res Advancement Inst, Kyoto 6158510, Japan

来源：

MACROMOLECULAR BIOSCIENCE | 2003年 / 3卷 / 12期

关键词：

enzyme inhibition; enzymatic polymerization; laccase; poly(catechin); superoxide radical scavenging; ENZYMATIC POLYMERIZATION; OXIDATIVE POLYMERIZATION; XANTHINE-OXIDASE; TEA EXTRACTS; (+)-CATECHIN OXIDATION; IN-VITRO; PEROXIDASE; CATECHIN; POLYPHENOLOXIDASE; METABOLITES;

D O I：

10.1002/mabi.200300038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Catechin was oxidatively polymerized by laccase in a mixture of a polar organic solvent and buffer to give a new class of flavonoid polymers. Myceliophthora laccase showed high catalytic activity for the polymerization. Using a mixed solvent of acetone and pH 5 acetate buffer efficiently produced the polymer. Under the selected conditions, DMF-soluble polymers were obtained in good yields. Effects of reaction parameters on the yield, solubility, and molecular weight of the polymer have been systematically investigated. A radical species was detected in the polymer by ESR spectroscopy. The polymers showed greatly amplified superoxide scavenging activity and xanthine oxidase inhibitory activity compared with monomeric catechin.

引用

页码：758 / 764

页数：7