Acute effect of ovariohysterectomy on lipid peroxidation and some antioxidant levels in dogs

The aim of this study was to investigate the acute effect of spaying on antioxidant defence mechanism and lipid peroxidation in healthy bitches. Thirty-one healthy bitches were used in the study. All the animals were anaesthetised with xylazine-ketamine combination and subjected to total ovariohysterectomy (OVH). The blood glutathione (GSH), plasma malondialdehyde (MDA), ceruloplasmin, beta-carotene, and retinol levels were determined in the preoperative and early postoperative periods. There was a significant decrease in blood GSH levels and an increase in plasma MDA activity at early postoperative stage as compared to the stage before the operation. There were no significant differences in the concentration of P-carotene, retinol, and ceruloplasmin levels between the preoperative and early postoperative periods. In conclusion, these findings indicate some alterations in the antioxidant defence system in the early postoperative stage. The assessment of GSH and MDA levels can be useful for the evaluation of oxidative stress after OVH in bitches.