Determination of protein structures - A series of fortunate events

被引：38
作者：
Chruszcz, Maksymilian [1 ]
Wlodawer, Alexander [2 ]
Minor, Wladek [1 ]
机构：
[1] Univ Virginia, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22903 USA
[2] NCI, Macromol Crystallog Lab, Prot Struct Sect, Frederick, MD 21701 USA
来源：
BIOPHYSICAL JOURNAL | 2008年 / 95卷 / 01期
关键词：
D O I：
10.1529/biophysj.108.131789
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Determination of a macromolecular structure using x-ray diffraction is a multistep process that involves a plethora of techniques involving molecular biology, bioinformatics, and physical sciences. Counterintuitively, the success of any or all individual steps does not guarantee the success of the overall process. This review examines the difficulties presented by each step on the path from a gene to the final publication, together with certain lucky (or unlucky) circumstances that can affect the velocity along that path.
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页码：1 / 9
页数：9
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