CBX7 regulates metastasis of basal-like breast cancer through Twist1/EphA2 pathway

被引：9
作者：
Dai, Tao [1 ]
Liu, Yiqi [2 ]
Cao, Renxian [3 ]
Cao, Jingying [4 ]
机构：
[1] Cent South Univ, Hunan Canc Hosp, Affiliated Canc Hosp, Dept Urol,Xiangya Sch Med, Changsha 410013, Hunan, Peoples R China
[2] Univ South China, Affiliated Hosp 2, Hengyang Med Sch, Hengyang 421001, Hunan, Peoples R China
[3] Univ South China, Affiliated Hosp 1, Hengyang Med Sch, Inst Clin Med, Hengyang 421001, Hunan, Peoples R China
[4] Cent South Univ, Xiangya Hosp 3, Dept Med Clin Lab, Changsha 410013, Hunan, Peoples R China
来源：
TRANSLATIONAL ONCOLOGY | 2022年 / 24卷
基金：
中国国家自然科学基金;
关键词：
Basal-like breast cancer; CBX7; EphA2; Metastasis; Twist1; TUMOR-METASTASIS; GROWTH; TARGET; EPHA2;
D O I：
10.1016/j.tranon.2022.101468
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: Basal-like breast cancer (BLBC) is an important subtype of breast cancer. Twist1 is a key transcription factor in BLBC metastasis, which serves a key role in tumorigenesis. The potential mechanism of Twist1 in BLBC remains to be elucidated. Here, we explored the role and molecular mechanism of Twist1 in BLBC. Methods: The levels of CBX7, Twist1 and EphA2 in BLBC tissues and cells were determined by Western blot. ChIP and dual-luciferase reporter assays confirmed the interaction between CBX7, Twist1 and EphA2 promoter. The cellular functions were analyzed by CCK-8, colony formation, wound healing and Transwell assays. Expression of EMT related proteins was analyzed by Western blot. IHC measured the expression of CBX7, Twist1 and EphA2 in tumor tissues. Results: CBX7 was down-regulated in BLBC tissues and cells, whereas Twist1 and EphA2 were up-regulated. Twist1 silencing inhibited the cell migration, invasion and cancer metastasis of BLBC through targeting EphA2 and regulating EphA2 expression. Additionally, CBX7 blocked the binding of Twist1 to EphA2 promoter and inhibited EphA2 expression and suppressed BLBC growth and metastasis via Twist1/EphA2 axis. Conclusion: CBX7 suppresses BLBC growth and metastasis through Twist1/EphA2 pathway. Our study may provide evidence and new therapeutic targets for the comprehensive treatment of BLBC.
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页数：12
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