The tomato I-3 gene: a novel gene for resistance to Fusarium wilt disease

被引:138
作者
Catanzariti, Ann-Maree [1 ]
Lim, Ginny T. T. [1 ]
Jones, David A. [1 ]
机构
[1] Australian Natl Univ, Div Plant Sci, Res Sch Biol, Acton, ACT 2601, Australia
基金
澳大利亚研究理事会;
关键词
effector-triggered immunity (ETI); fungal pathogen; Fusarium wilt; plant resistance genes; Solanum lycopersicum (tomato); S-receptor-like kinase (SRLK); RECEPTOR-LIKE KINASE; SELF-INCOMPATIBILITY; NUCLEOTIDE-BINDING; I-3-MEDIATED RESISTANCE; HYPERSENSITIVE RESPONSE; BRASSICA-OLERACEA; ARABIDOPSIS; EXPRESSION; OXYSPORUM; LOCUS;
D O I
10.1111/nph.13348
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plant resistance proteins provide race-specific immunity through the recognition of pathogen effectors. The resistance genes I, I-2 and I-3 have been incorporated into cultivated tomato (Solanum lycopersicum) from wild tomato species to confer resistance against Fusarium oxysporum f. sp. lycopersici (Fol) races 1, 2 and 3, respectively. Although the Fol effectors corresponding to these resistance genes have all been identified, only the I-2 resistance gene has been isolated from tomato. To isolate the I-3 resistance gene, we employed a map-based cloning approach and used transgenic complementation to test candidate genes for resistance to Fol race 3. Here, we describe the fine mapping and sequencing of genes at the I-3 locus, which revealed a family of S-receptor-like kinase (SRLK) genes. Transgenic tomato lines were generated with three of these SRLK genes and one was found to confer Avr3-dependent resistance to Fol race 3, confirming it to be I-3. The finding that I-3 encodes an SRLK reveals a new pathway for Fol resistance and a new class of resistance genes, of which Pi-d2 from rice is also a member. The identification of I-3 also allows the investigation of the complex effector-resistance protein interaction involving Avr1-mediated suppression of I-2- and I-3-dependent resistance in tomato.
引用
收藏
页码:106 / 118
页数:13
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