Large-scale solution synthesis of phosphorothioate oligonucleotides: A comparison of the phosphoroamidite and phosphotriester dimeric approaches

Two different approaches to the solution synthesis of oligonucleotides are compared. Both procedures use dimer synthons as building blocks to save time and to improve the final product yield and quality. These strategies look quite promising due to their easy upscaling, providing an advantageous alternative to the well-established solid-phase methods.