Determination of folic acid in fortified wheat flours

被引：60
|
作者：
Alaburda, Janete [1 ]
de Almelda, Adriana P. [1 ]
Shundo, Luzia [1 ]
Ruvieri, Valter [1 ]
Sabino, Myrna [1 ]
机构：
[1] Inst Adolfo Lutz Registro, Secao Quim Biol, BR-01246902 Sao Paulo, Brazil
来源：
JOURNAL OF FOOD COMPOSITION AND ANALYSIS | 2008年 / 21卷 / 04期
关键词：
folic acid; fortification; wheat flour; HPLC; ELISA; Sao Paulo; Brazil; regulation; microbiological assay; immunoenzymatic assay;
D O I：
10.1016/j.jfca.2007.12.002
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A reversed-phase high-performance liquid chromatography (HPLC) method with ultraviolet detection to determine folic acid (FA) in fortified wheat flour is described. The method includes FA extraction with tetraborate and trichloroacetic acid buffer solution and purification by solid-phase extraction with strong anion-exchange cartridges. Good results were obtained with respect to repeatability (relative standard deviation <= 8.8) and recovery (>= 97%). Detection and quantification limits were 0.06 and 0.19 mu g g(-1), respectively. The comparison between the HPLC method and both microbiological and immunoenzymatic assays revealed similar results. FA determination results on 33 wheat flours samples revealed that 51% of these samples had below 1.50 mu g g(-1) FA concentration added, the enrichment level established by Brazilian Regulation. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码：336 / 342
页数：7
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