Reduced ultraviolet-induced DNA damage and apoptosis in human skin with topical application of a photolyase-containing DNA repair enzyme cream: Clues to skin cancer prevention

被引:61
作者
Berardesca, Enzo [2 ]
Bertona, Marco [1 ]
Altabas, Karmela [3 ]
Altabas, Velimir [3 ]
Emanuele, Enzo [1 ]
机构
[1] Univ Pavia, Dept Hlth Sci, I-27100 Pavia, Italy
[2] IRCCS, San Gallicano Dermatol Inst, Rome, Italy
[3] Univ Zagreb, Clin Hosp Sestre Milosrdnice, Dept Internal Med, HR-10002 Zagreb, Croatia
关键词
photolyase; ultraviolet radiation; skin; cyclobutane-type pyrimidine dimers; apoptosis; skin cancer; CYCLOBUTANE PYRIMIDINE DIMERS; LIGHT; PROTECTION; PHOTOREACTIVATION; PHOTOPROTECTION; KERATINOCYTES; CONSEQUENCES; PATHOGENESIS; SUNSCREEN; THERAPY;
D O I
10.3892/mmr.2011.673
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The exposure of human skin to ultraviolet radiation (UVR) results in the formation of DNA photolesions that give rise to photoaging, mutations, cell death and the onset of carcinogenic events. Photolyase (EC 4.1.99.3) is a DNA repair enzyme that reverses damage caused by exposure to UVR. We sought to investigate whether addition of photolyase enhances the protection provided by a traditional sunscreen (SS), by reducing the in vivo formation of cyclobutane-type pyrimidine dimers (CPDs) and UVR-induced apoptosis in human skin. Ten volunteers (Fitzpatrick skin type II) were exposed to solar-simulated (ss) UVR at a three times minimal erythema dose for 4 consecutive days. Thirty minutes prior to each exposure, the test materials [vehicle, SS (sun protection factor 50) alone, and SS plus photolyase from Anacystis nidulans] were applied topically to three different sites. One additional site was left untreated and one received ssUVR only. Biopsy specimens were taken 72 h after the last irradiation. The amount of CPDs and the extent of apoptosis were measured by ELISA. Photolyase plus SS was superior to SS alone in reducing both the formation of CPDs and apoptotic cell death (both P<0.001). In conclusion, the addition of photolyase to a traditional SS contributes significantly to the prevention of UVR-induced DNA damage and apoptosis when applied topically to human skin.
引用
收藏
页码:570 / 574
页数:5
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