BzDANP, a Small-Molecule Modulator of Pre-miR-29a Maturation by Dicer

被引：19

作者：

Murata, Asako ^{[1
]}

Otabe, Takahiro ^{[1
]}

Zhang, Jinhua ^{[1
]}

Nakatani, Kazuhiko ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Dept Regulatory Bioorgan Chem, 8-1 Mihogaoka, Osaka 5670047, Japan

来源：

ACS CHEMICAL BIOLOGY | 2016年 / 11卷 / 10期

关键词：

GUANINE-GUANINE MISMATCHES; SURFACE-PLASMON RESONANCE; HELIX-THREADING PEPTIDES; TARGETING RNA; NONCODING RNAS; IN-VIVO; ONCOGENIC MICRORNAS; NUCLEIC-ACIDS; HIGH-AFFINITY; DIMERIC FORM;

D O I：

10.1021/acschembio.6b00214

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We here report the synthesis of novel molecule BzDANP having a three-ring benzo[c][1,8]naphthyridine system, the evaluation of its binding properties to a single nucleotide bulge in RNA duplexes, and BzDANP-induced suppression of pre-miR-29a processing by Dicer. BzDANP showed much increased affinity to the bulged RNAs as compared with the parent molecule DANP, which possesses the same hydrogen-bonding surface as BzDANP but in a two-ring [1,8]naphthyridine system. Melting temperature analysis of bulged RNAs showed that BzDANP most effectively stabilized the C-bulged RNA. Dicer-mediated processing of pre-miR-29a was suppressed by BzDANP in a concentration dependent manner. The presence of the C-bulge at the Dicer cleavage site was effective for the suppression of pre-miR-29a processing by BzDANP. These results demonstrated that the small molecule binding to the bulged site in the vicinity of the Dicer cleavage site could be a potential modulator for the maturation of pre-miRNA.

引用

页码：2790 / 2796

页数：7

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