Modular polyketide synthases: Investigating intermodular communication using 6 deoxyerythronolide B synthase module 2

被引：4

作者：

Moffet, DA

Khosla, C

Cane, DE

机构：

[1] Brown Univ, Dept Chem, Providence, RI 02912 USA

[2] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Chem & Biochem, Stanford, CA 94305 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2006年 / 16卷 / 01期

关键词：

biosynthesis; polyketides; molecular recognition;

D O I：

10.1016/j.bmcl.2005.09.017

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A novel variant of 6-deoxyerythronolide B synthase (DEBS) module 2 was constructed to explore the balance between protein-protein-mediated intermodular channeling and intrinsic substrate specificity within DEBS. This construct, termed (N3)Mod2 + TE, was co-incubated with a complementary, donor form of the same module, (N5)Mod2(C2), as well as with a mutant of (N5)Mod2(C2) with an inactive ketosynthase domain, in order to determine the extent of intermediate channeling versus substrate diffusion into the downstream module. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：213 / 216

页数：4