Correlations between gastric cancer family history and ROBO2 and RASSF2A gene methylations

被引：11
作者：
Chang, Zhi-Wei [1 ]
Dong, Lei [1 ]
Qin, Yan-Ru [1 ]
Song, Min [1 ]
Guo, Hai-Yun [2 ]
Zhu, Qing-Li [2 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Oncol, Zhengzhou, Peoples R China
[2] Linzhou Ctr Hosp, Dept Pathol, Linzhou, Peoples R China
来源：
JOURNAL OF CANCER RESEARCH AND THERAPEUTICS | 2016年 / 12卷 / 02期
关键词：
Gastric cancer; methylation; family history; RPBO2; RASSF2A; PROMOTER HYPERMETHYLATION; TUMOR-SUPPRESSOR; EFFECTOR; EXPRESSION; MUTATIONS;
D O I：
10.4103/0973-1482.146089
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Objective: To explore the correlation between ROBO2 and RASSF2A gene methylations and gastric cancer family history. Materials and Methods: ROBO2 and RASSF2A gene methylations in gastric cancer tissues and peri.cancerous tissues were detected with methylation.specific PCR in 36. patients with gastric cancer family history and 33 without gastric cancer family history. The correlations of ROBO2 and RASSF2A gene methylations with family history, and clinical and pathological characteristics were analyzed. Results: ROBO2 and RASSF2A gene methylations were all significantly higher in gastric cancer tissues (30% and 26%) than in peri-cancerous tissues (0% and 0%) (all P 0.05). ROBO2 gene methylation was significantly lower in the patients with gastric cancer family history (17%, 6/36) than in the patients without gastric cancer family history (41%, 15/33) (P 0.05). Conclusion: ROBO2 and RASSF2A gene methylations may be related to gastric tumorigenesis, and ROBO2 gene methylation is associated with sporadic gastric cancer.
引用
收藏
页码：597 / 600
页数：4
相关论文
共 21 条
[1]   The Ras effector RASSF2 is a novel tumor-suppressor gene in human colorectal cancer [J].
Akino, K ;
Toyota, M ;
Suzuki, H ;
Mita, H ;
Sasaki, Y ;
Ohe-Toyota, M ;
Issa, JPJ ;
Hinoda, Y ;
Imai, K ;
Tokino, T .
GASTROENTEROLOGY, 2005, 129 (01) :156-169
[2]  
[Anonymous], 2018, ANTI-CANCER DRUG, DOI [DOI 10.3322/caac.20115, DOI 10.1097/CAD.0000000000000617]
[3]   Down-regulation of ROBO2 Expression in Prostate Cancers [J].
Choi, Youn Jin ;
Yoo, Nam Jin ;
Lee, Sug Hyung .
PATHOLOGY & ONCOLOGY RESEARCH, 2014, 20 (03) :517-519
[4]   Epigenetics in cancer: Targeting chromatin modifications [J].
Ellis, Leigh ;
Atadja, Peter W. ;
Johnstone, Ricky W. .
MOLECULAR CANCER THERAPEUTICS, 2009, 8 (06) :1409-1420
[5]   Alterations of ROBO1/DUTT1 and ROBO2 loci in early dysplastic lesions of head and neck: clinical and prognostic implications [J].
Ghosh, Susmita ;
Ghosh, Amlan ;
Maiti, Guru Prasad ;
Alam, Neyaz ;
Roy, Anup ;
Roychoudhury, Susanta ;
Panda, Chinmay Kumar .
HUMAN GENETICS, 2009, 125 (02) :189-198
[6]   CpG island promoter hypermethylation of a novel Ras-effector gene RASSF2A is an early event in colon carcinogenesis and correlates inversely with K-ras mutations [J].
Hesson, LB ;
Wilson, R ;
Morton, D ;
Adams, C ;
Walker, M ;
Maher, ER ;
Latif, F .
ONCOGENE, 2005, 24 (24) :3987-3994
[7]   CpG islands - 'A rough guide' [J].
Illingworth, Robert S. ;
Bird, Adrian P. .
FEBS LETTERS, 2009, 583 (11) :1713-1720
[8]   Frameshift mutations of axon guidance genes ROBO1 and ROBO2 in gastric and colorectal cancers with microsatellite instability [J].
Je, Eun Mi ;
Gwak, Min ;
Oh, Hyerim ;
Choi, Mi Ryoung ;
Choi, Youn Jin ;
Lee, Sug Hyung ;
Yoo, Nam Jin .
PATHOLOGY, 2013, 45 (07) :645-650
[9]   Aggregation of stomach cancer history in parents and offspring in comparison with other sites [J].
Kondo, T ;
Toyoshima, H ;
Tsuzuki, Y ;
Hori, Y ;
Yatsuya, H ;
Tamakoshi, K ;
Tamakoshi, A ;
Ohno, Y ;
Kikuchi, S ;
Sakata, K ;
Hoshiyama, Y ;
Hayakawa, N ;
Tokui, N ;
Mizoue, T ;
Yoshimura, T .
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY, 2003, 32 (04) :579-583
[10]   E-cadherin gene (CDH1) promoter methylation as the second hit in sporadic diffuse gastric carcinoma [J].
Machado, JC ;
Oliveira, C ;
Carvalho, R ;
Soares, P ;
Berx, G ;
Caldas, C ;
Seruca, R ;
Carneiro, F ;
Sobrinho-Simoes, M .
ONCOGENE, 2001, 20 (12) :1525-1528
123