Heterogeneous IgE reactivities to Staphylococcus pseudintermedius strains in dogs with atopic dermatitis, and the identification of DM13-domain-containing protein as a bacterial IgE-reactive molecule

被引：1

作者：

Takemura-Uchiyama, Iyo ^{[1
,2
]}

Tsurui, Hiroki ^{[2
]}

Shimakura, Hidekatsu ^{[2
]}

Nasukawa, Tadahiro ^{[2
]}

Imanishi, Ichiro ^{[2
,3
]}

Uchiyama, Jumpei ^{[1
,2
]}

Fukuyama, Tomoki ^{[2
]}

Sakamoto, Shuji ^{[4
]}

Morisawa, Keiko ^{[4
]}

Fujimura, Masato ^{[5
]}

Murakami, Hironobu ^{[2
]}

Kanamaru, Shuji ^{[6
]}

Kurokawa, Kenji ^{[7
]}

Kawamoto, Keiko ^{[2
]}

Iyori, Keita ^{[8
]}

Sakaguchi, Masahiro ^{[2
,9
]}

机构：

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Bacteriol, Kita Ku, Shikata Cho 2-5-1, Okayama, Okayama 7008558, Japan

[2] Azabu Univ, Sch Vet Med, Chuo Ku, Fuchinobe 1-17-71, Sagamihara, Kanagawa 2525201, Japan

[3] Kitasato Univ, Sch Med, Minami Ku, Kitasato 1-15-1, Sagamihara, Kanagawa 2520373, Japan

[4] Kochi Med Sch, Sci Res Ctr, Oko Cho 185-1 Kohasu, Nankoku, Kochi 7838505, Japan

[5] Fujimura Anim Hosp, Aomadanihigashi 5-10-26, Mino, Osaka 5620022, Japan

[6] Tokyo Inst Technol, Dept Life Sci & Technol, Midori Ku, Nagatsuda Cho 4259, Yokohama, Kanagawa 2260026, Japan

[7] Nagasaki Int Univ, Fac Pharmaceut Sci, Huistenbosch Cho 2825-7, Nagasaki 8593298, Japan

[8] Vet Derm Tokyo, Dermatol & Lab Serv Anim, Fujisawa, Kanagawa 2520823, Japan

[9] Inst Tokyo Environm Allergy, Bunkyo Ku, Hakusan 1-33-18, Tokyo 1130001, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2022年 / 369卷 / 01期

关键词：

Staphylococcus pseudintermedius; atopic dermatitis; IgE; dogs; DM13-domain-containing protein; exacerbation factor; EPIDEMIOLOGY; TOXINS;

D O I：

10.1093/femsle/fnac019

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Staphylococcus pseudintermedius is one of the major pathogens causing canine skin infection. In canine atopic dermatitis (AD), heterogeneous strains of S. pseudintermedius reside on the affected skin site. Because an increase in specific IgE to this bacterium has been reported, S. pseudintermedius is likely to exacerbate the severity of canine AD. In this study, the IgE reactivities to various S. pseudintermedius strains and the IgE-reactive molecules of S. pseudintermedius were investigated. First, examining the IgE reactivities to eight strains of S. pseudintermedius using 141 sera of AD dogs, strain variation of S. pseudintermedius showed 10-63% of the IgE reactivities. This is different from the expected result based on the concept of Staphylococcus aureus clonality in AD patients. Moreover, according to the western blot analysis, there were more than four proteins reactive to IgE. Subsequently, the analysis of the common IgE-reactive protein at similar to 15 kDa confirmed that the DM13-domain-containing protein was reactive in AD dogs, which is not coincident with any S. aureus IgE-reactive molecules. Considering these, S. pseudintermedius is likely to exacerbate AD severity in dogs, slightly different from the case of S. aureus in human AD. IgE reactivities to Staphylococcus pseudintermedius.

引用

页数：7