Detection of Streptobacillus spp. in feral rats by specific polymerase chain reaction

被引：37

作者：

Kimura, Masanobu ^{[1
]}

Tanikawa, Tsutomu ^{[3
]}

Suzuki, Michio ^{[1
]}

Koizumi, Nobuo ^{[2
]}

Kamiyama, Tsuneo ^{[1
]}

Imaoka, Koichi ^{[1
]}

Yamada, Akio ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Vet Sci, Shinjuku Ku, Tokyo 1628640, Japan

[2] Natl Inst Infect Dis, Dept Bacteriol, Tokyo 1628640, Japan

[3] Ikari Corp, Chiba, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2008年 / 52卷 / 01期

关键词：

16S rRNA gene; polymerase chain reaction; rat-bite fever; Streptobacillus spp;

D O I：

10.1111/j.1348-0421.2008.00005.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Streptobacillus moniliformis is an etiological agent of rat-bite fever and Haverhill fever in human infection. As the currently available methods for identifying the causative bacteria are not satisfactory, we attempted to establish them by PCR using newly designed primers for the 16S rRNA gene of S. moniliformis. We then determined the prevalence of Streptobacillus spp. in two species of feral rats that inhabit an urban region in Japan, because information on the prevalence of the bacteria in feral rats is obscure. The use of PCR with newly designed primers showed that an extremely high proportion of R. norvegicus harbored the bacteria (61/66, 92%), whereas the prevalence was only 58% in R. rattus (30/52). The nucleotide sequence analysis of the 16S rRNA gene of Streptobacillus spp. isolated from oral swabs of feral rats showed at least two different types of bacteria among isolates from R. norvegicus and R. rattus.

引用

页码：9 / 15

页数：7

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