Anti-inflammatory and anti-fibrotic effects of intravenous adipose-derived stem cell transplantation in a mouse model of bleomycin-induced interstitial pneumonia

被引：56

作者：

Kotani, Takuya ^{[1
]}

Masutani, Ryota ^{[3
]}

Suzuka, Takayasu ^{[1
]}

Oda, Katsuhiro ^{[1
]}

Makino, Shigeki ^{[1
]}

Ii, Masaaki ^{[2
]}

机构：

[1] Osaka Med Coll, Dept Internal Med 4, Osaka, Japan

[2] Osaka Med Coll, Res & Dev Ctr, Div Res Anim Lab & Translat Med, Osaka, Japan

[3] Osaka Med Coll, Div Cent Lab, Osaka, Japan

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

CONDITIONED MEDIUM; LUNG INJURY; THERAPY; FIBROSIS; TISSUE; ENGRAFTMENT; MICE;

D O I：

10.1038/s41598-017-15022-3

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Adipose-derived stem cells (AdSCs) have recently been considered a useful treatment tool for autoimmune disease because of their anti-inflammatory and immunosuppressive effects. We investigated the therapeutic effect of intravenous AdSC transplantation in a mouse model of bleomycin-induced lung injury. AdSCs accumulated in the pulmonary interstitium and inhibited both inflammation and fibrosis in the lung, markedly improving the survival rate of mice with bleomycin-induced lung injury in a cell number-dependent manner. AdSCs inhibited the production of proinflammatory cytokines such as TNF-alpha and IL-12 in activated macrophages, and AdSCs also induced the apoptosis of activated macrophages. AdSCs inhibited the differentiation and proliferation of Th2-type mCD4+ T cells but promoted the differentiation and proliferation of regulatory T cells, suggesting that the phenotypic conversion of T cells may be one of the mechanisms for the anti-inflammatory effect of AdSCs on pulmonary fibrosis. These findings suggest that intravenous AdSCs could be a promising treatment for patients with interstitial pneumonia.

引用

页数：10

共 42 条

[1] Bone Marrow Stem Cells Expressing Keratinocyte Growth Factor via an Inducible Lentivirus Protects against Bleomycin-Induced Pulmonary Fibrosis [J].