An injectable gellan gum-based hydrogel that inhibits Staphylococcus aureus for infected bone defect repair

被引：25
作者：
Xu, Laijun [1 ,2 ,3 ,4 ]
Ye, Qing [1 ,2 ,3 ]
Xie, Jing [5 ]
Yang, Jiaojiao [1 ,2 ,3 ]
Jiang, Wentao [6 ]
Yuan, He [1 ,2 ,3 ]
Li, Jiyao [1 ,2 ,3 ]
机构：
[1] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China
[2] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu 610041, Peoples R China
[3] Sichuan Univ, West China Hosp Stomatol, Dept Cariol & Endodont, Chengdu 610041, Peoples R China
[4] Cent South Univ, Xiangya Stomatol Hosp, Xiangya Sch Stomatol, Dept Operat Dent & Endodont, Changsha 410008, Peoples R China
[5] Sichuan Univ, Coll Polymer Sci & Engn, Chengdu 610065, Peoples R China
[6] Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Guangdong Prov Key Lab Stomatol, Guangzhou 510060, Peoples R China
来源：
JOURNAL OF MATERIALS CHEMISTRY B | 2022年 / 10卷 / 02期
基金：
中国国家自然科学基金;
关键词：
BACTERIAL-INFECTION; CELL; HYDROXYAPATITE; MANAGEMENT;
D O I：
10.1039/d1tb02230j
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
The treatment of infected bone defects in complex anatomical structures, such as oral and maxillofacial structures, remains an intractable clinical challenge. Therefore, advanced biomaterials that have excellent anti-infection activity and allow convenient delivery are needed. We fabricated an innovative injectable gellan gum (GG)-based hydrogel loaded with nanohydroxyapatite particles and chlorhexidine (nHA/CHX). The hydrogel has a porous morphology, suitable swelling ratio, and good biocompatibility. It exerts strong antibacterial activity against Staphylococcus aureus growth and biofilm formation in vitro. We successfully established an infected calvarial defect rat model. Bacterial colony numbers were significantly lower in tissues surrounding the bone in rats of the GG/nHA/CHX group after debride surgery and hydrogel implantation in the defect regions than in rats of the blank group. Rats in the GG/nHA/CHX group exhibited significantly increased new bone formation compared to those in the blank group at 4 and 8 weeks. These findings indicate that gellan gum-based hydrogel with nHA/CHX can accelerate the repair of infected bone defects.
引用
收藏
页码：282 / 292
页数：11
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