High-throughput yeast two-hybrid assays for large-scale protein interaction manning

被引：201

作者：

Walhout, AJM

Vidal, M ^{[1
]}

机构：

[1] Dana Farber Canc Inst, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

来源：

METHODS | 2001年 / 24卷 / 03期

关键词：

D O I：

10.1006/meth.2001.1190

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein-protein interactions play fundamental roles in many biological processes. Hence, protein interaction mapping is becoming a well-established functional genomics approach to generate functional annotations for predicted proteins that so far have remained uncharacterized. The yeast two-hybrid system is currently one of the most standardized protein interaction mapping techniques. Here, we describe the protocols for a semiautomated, high-throughput, Gal4-based yeast two-hybrid system. (C) 2001 Academic Press.

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页码：297 / 306

页数：10

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