Genetic characterization of Toxoplasma gondii in meat-producing animals in Iran

被引：6

作者：

Amouei, Afsaneh ^{[1
,2
]}

Sarvi, Shahabeddin ^{[1
,2
]}

Mizani, Azadeh ^{[3
]}

Hashemi-Soteh, Mohammad Bagher ^{[4
]}

Salehi, Saeid ^{[5
]}

Javidnia, Javad ^{[6
]}

Hosseini, Seyed Abdollah ^{[1
,2
]}

Amuei, Fateme ^{[7
]}

Alizadeh, Ahad ^{[8
]}

Shabanzade, Shafigheh ^{[9
]}

Gholami, Sara ^{[2
]}

Daryani, Ahmad ^{[1
,2
]}

机构：

[1] Mazandaran Univ Med Sci, Toxoplasmosis Res Ctr, Communicable Dis Inst, Sari, Iran

[2] Mazandaran Univ Med Sci, Sch Med, Dept Parasitol, Sari 4847191971, Mazandaran, Iran

[3] Pasteur Inst Iran, Dept Parasitol, Tehran, Iran

[4] Mazandaran Univ Med Sci, Fac Med, Dept Clin Biochem & Genet, Sari, Iran

[5] Mazandaran Prov Vet Dept Med Sci, Sari, Iran

[6] Mazandaran Univ Med Sci, Sch Med, Dept Mycol, Sari, Iran

[7] Univ Mazandaran, Dept Organ Chem, Babolsar, Iran

[8] Qazvin Univ Med Sci, Metab Dis Res Ctr, Res Inst Prevent Noncommunicable Dis, Qazvin, Iran

[9] Shiraz Univ Med Sci, Sch Med, Dept Parasitol & Mycol, Shiraz, Iran

来源：

PARASITES & VECTORS | 2022年 / 15卷 / 01期

基金：

欧洲研究理事会;

关键词：

Toxoplasma gondii; Genotype; Diversity; Meat-producing animals; Mazandaran; Iran; POLYMERASE-CHAIN-REACTION; POPULATION-STRUCTURE; MOLECULAR-DETECTION; SEROLOGICAL SURVEY; SHEEP; INFECTION; GOATS; SEROPREVALENCE; IDENTIFICATION; PREVALENCE;

D O I：

10.1186/s13071-022-05360-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background: The consumption of uncooked or undercooked food from infected intermediate hosts can result in Toxoplasma gondii infection in humans. However, few studies have investigated the genetic diversity of this protozoan parasite in Iran. The aim of the present study was to genetically characterize isolates of T. gondii from intermediate host animals in Mazandaran Province, Iran. Methods: Blood and heart tissue samples were collected from 204 ruminants, and brain tissue was collected from 335 birds. The prevalence of T. gondii infection in these samples was determined serologically using the modified agglutination test and by conventional PCR assays. Those PCR samples positive for T.gondii DNA and 13 DNA samples extracted from aborted fetuses in a previous study were genotyped with 12 genetic markers using the multilocusnested PCR-restriction fragment length polymorphism (Mn-PCR-RFLP) technique. Results: Antibodies for parasites were found in 35.7% of the ruminant (39.1% of sheep and 26.4% of goats) samples and in 51.3% of the bird (100% of geese, 52.3% of free-range chickens and 46% of ducks) samples. Molecular detection by PCR of the repetitive 529-bp DNA fragment revealed contamination of 13.2% of ruminant (14.6% of sheep and 9.4% of goats) samples and of 9.6% of bird (11.1% of chickens, 5.7% of ducks and 0% of geese samples). The results from 30 DNA samples revealed five ToxoDB (genome database for the genus Toxoplasma) PCR-RFLP genotypes, including #1 (Type II), #2 (Type III), #10 (Type I), #27 and #48, with genotype #1 the most predominant. Conclusions: As evidenced by the results of this study, ruminants and birds are infected with T. gondii in the region, suggesting that they are likely to be involved in the transmission of T. gondii to humans through meat consumption. The identification of different genotypes may suggest a higher genetic diversity of this parasite in Mazandaran, reflecting local environmental contamination. These results have important public health implications for the prevention and control strategies of infection.

引用

页数：11

共 58 条

[21]

Fereig Ragab M, 2016, Vet Parasitol Reg Stud Reports, V3-4, P1, DOI 10.1016/j.vprsr.2016.05.002

[22] A Systematic Meta-Analysis of Toxoplasma gondii Prevalence in Food Animals in the United States [J].