Effects of dihydrotestosterone on brain mRNA levels of steroid 5α-reductase isozymes in early postnatal life of rat

被引：14
作者：
Sánchez, P
Torres, JM
Ortega, E
机构：
[1] Univ Granada, Fac Med, Dept Biochem & Mol Biol, Granada 18012, Spain
[2] Univ Granada, Inst Neurosci, Granada, Spain
来源：
NEUROCHEMICAL RESEARCH | 2005年 / 30卷 / 04期
关键词：
5 alpha-reductase type 1; 5 alpha-reductase type 2; transcriptional regulation; prefrontal cortex; early postnatal life; dihydrotestosterone; male rat; female rat; one-step quantitative RT-PCR; fluorescence capillary electrophoresis; central nervous system; sexual differentiation; sexual dimorphism; mRNA expression; competitor DNA; plasmid pEGFP-C1; cDNA; oligonucleotides;
D O I：
10.1007/s11064-005-2692-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We studied the expression of type 1 (5 alpha-R1) and type 2 (5 alpha-R2) 5 alpha-reductase isozymes (5 alpha-R) and their regulation by dihydrotestosterone (DHT) in the prefrontal cortex of male and female rats during postnatal sexual differentiation of the central nervous system (CNS), using one-step quantitative RT-PCR coupled with laser-induced fluorescence capillary electrophoresis. We found a higher expression of 5 alpha-R2, which is considered a masculinizing enzyme, in the female versus male CNS, and observed sexual dimorphism in the regulation of both 5 alpha-R isozymes by DHT. These results open up a new research line that could improve understanding of the role of 5 alpha-R isozymes in the physiology of the CNS.
引用
收藏
页码：577 / 581
页数：5
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