The extra-intestinal avian pathogenic Escherichia coli strain BEN2908 invades avian and human epithelial cells and survives intracellularly

被引：25

作者：

Chanteloup, Nathalie Katy ^{[1
]}

Porcheron, Gaelle ^{[1
]}

Delaleu, Bernadette ^{[2
]}

Germon, Pierre ^{[1
]}

Schouler, Catherine ^{[1
]}

Moulin-Schouleur, Maryvonne ^{[1
]}

Gilot, Philippe ^{[1
]}

机构：

[1] INRA, Lab Pathogenie Bacterienne, Infectiol Anim & Sante Publ UR1282, F-37380 Nouzilly, France

[2] Univ Tours, INRA, CNRS, Physiol Reprod & Comportements UMR85, F-37380 Nouzilly, France

来源：

VETERINARY MICROBIOLOGY | 2011年 / 147卷 / 3-4期

关键词：

ExPEC; APEC; Intracellular; Epithelial cells; Type; 1; fimbriae; Zoonotic risk; MEDIATED BACTERIAL INVASION; HOST-CELLS; COLONIZATION; ESTABLISHMENT; CHICKENS; ENTRY;

D O I：

10.1016/j.vetmic.2010.07.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Extra-intestinal pathogenic Escherichia coli (ExPEC) strains are responsible for a wide range of diseases in humans and animals. Using in vitro invasion assays and transmission electron microscopy, we showed that BEN2908, an ExPEC strain of avian origin (also termed APEC for Avian Pathogenic E. coli), is able to usurp cellular endocytic pathways to invade A549 human type II pneumocytes and LMH avian hepatocytes where it is able to survive over several days. Although type 1 fimbriae are the major adhesin of BEN2908, proportions of adherent fimbriated or afimbriated bacteria that entered cells were comparable. Internalization of BEN2908 into human pneumocytes reinforces previous studies indicating that APEC strains could represent a zoonotic risk. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：435 / 439

页数：5

共 18 条

[1] Signature-Tagged Mutagenesis in a Chicken Infection Model Leads to the Identification of a Novel Avian Pathogenic Escherichia coli Fimbrial Adhesin [J].

Antao, Esther-Maria ;

Ewers, Christa ;

Guerlebeck, Doreen ;

Preisinger, Rudolf ;

Homeier, Timo ;

Li, Ganwu ;

Wieler, Lothar H. .

PLOS ONE, 2009, 4 (11)

[2] Survival of FimH-expressing enterobacteria in macrophages relies on glycolipid traffic [J].

Baorto, DM ;

Gao, ZM ;

Malaviya, R ;

Dustin, ML ;

vanderMerwe, A ;

Lublin, DM ;

Abraham, SN .

NATURE, 1997, 389 (6651) :636-639

[3] ESCHERICHIA-COLI COLONIZATION OF THE TRACHEA IN POULTRY - COMPARISON OF VIRULENT AND AVIRULENT STRAINS IN GNOTOXENIC CHICKENS [J].

DHO, M ;

LAFONT, JP .

AVIAN DISEASES, 1982, 26 (04) :787-797

[4] BACTERIAL-COLONIZATION AND IN-VIVO EXPRESSION OF F1 (TYPE-1) FIMBRIALO ANTIGENS IN CHICKENS EXPERIMENTALLY INFECTED WITH PATHOGENIC ESCHERICHIA-COLI [J].

DOZOIS, CM ;

CHANTELOUP, N ;

DHOMOULIN, M ;

BREE, A ;

DESAUTELS, C ;

FAIRBROTHER, JM .

AVIAN DISEASES, 1994, 38 (02) :231-239

[5] Bacterial penetration of bladder epithelium through lipid rafts [J].

Duncan, MJ ;

Li, GJ ;

Shin, JS ;

Carson, JL ;

Abraham, SN .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (18) :18944-18951

[6] Clathrin, AP-2, and the NPXY-binding subset of alternate endocytic adaptors facilitate FimH-mediated bacterial invasion of host cells [J].

Eto, Danelle S. ;

Gordon, Hannah B. ;

Dhakal, Bijaya K. ;

Jones, Tiffani A. ;

Mulvey, Matthew A. ;

Gordon, Hannah B. ;

Dhakal, Bijaya K. .

CELLULAR MICROBIOLOGY, 2008, 10 (12) :2553-2567

[7] IN-VITRO CULTIVATION OF HUMAN TUMORS - ESTABLISHMENT OF CELL LINES DERIVED FROM A SERIES OF SOLID TUMORS [J].

GIARD, DJ ;

AARONSON, SA ;

TODARO, GJ ;

ARNSTEIN, P ;

KERSEY, JH ;

DOSIK, H ;

PARKS, WP .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1973, 51 (05) :1417-1423

[8]

Giuseppe S.A. Longo-Sorbello., 2006, Cytotoxicity and Cell Growth Assays, cell and tissue culture: assorted techniques, P315, DOI [DOI 10.1016/B978-012164730-8/50039-3, 10.1016/B978-012164730-8/50039-3]

[9] Curli fibers mediate internalization of Escherichia coli by eukaryotic cells [J].

Gophna, U ;

Barlev, M ;

Seijffers, R ;

Oelschlager, TA ;

Hacker, J ;

Ron, EZ .

INFECTION AND IMMUNITY, 2001, 69 (04) :2659-2665

[10]

KAWAGUCHI T, 1987, CANCER RES, V47, P4460

← 1 2 →