Mesenchymal Stem Cell Exosomes Derived from Feline Adipose Tissue Enhance the Effects of Anti-Inflammation Compared to Fibroblasts-Derived Exosomes

被引：6

作者：

Sung, Soo-Eun ^{[1
,2
]}

Seo, Min-Soo ^{[1
]}

Kang, Kyung-Ku ^{[1
]}

Choi, Joo-Hee ^{[1
]}

Lee, Sijoon ^{[1
]}

Sung, Minkyoung ^{[1
]}

Kim, Kilsoo ^{[1
,3
]}

Lee, Gun Woo ^{[4
]}

Lim, Ju-Hyeon ^{[4
,5
]}

Yang, Seung Yun ^{[2
]}

Yim, Sang-Gu ^{[2
]}

Kim, Seul-Ki ^{[6
]}

Park, Sangbum ^{[7
,8
,9
]}

Kwon, Young-Sam ^{[10
]}

Yun, Sungho ^{[10
]}

机构：

[1] Daegu Gyeongbuk Med Innovat Fdn DGMIF, Lab Anim Ctr, Daegu 41061, South Korea

[2] Pusan Natl Univ, Dept Biomat Sci, BK21 Program 4, Miryang 50463, South Korea

[3] Kyungpook Natl Univ, Coll Vet Med, 80 Daehakro, Daegu 41566, South Korea

[4] Yeungnam Univ Coll Med, Yeungnam Univ Med Ctr, Dept Orthoped Surg, 170 Hyochung Ro, Daegu 42415, South Korea

[5] Osong Med Innovat Fdn, New Drug Dev Ctr, Chungbuk 28160, South Korea

[6] KolmarBNH CO LTD, Food Sci R&D Ctr, Efficacy Evaluat Team, 61 Heolleungro 8-Gil, Seoul 06800, South Korea

[7] Michigan State Univ, Inst Quantitat Hlth Sci & Engn IQ, Auditorium Rd 775 Woodlot Dr, E Lansing, MI 48824 USA

[8] Michigan State Univ, Coll Human Med, Dept Med, Div Dermatol, Auditorium Rd 775 Woodlot Dr, E Lansing, MI 48824 USA

[9] Michigan State Univ, Coll Human Med, Dept Pharmacol & Toxicol, 775 Woodlot Dr, E Lansing, MI 48824 USA

[10] Kyungpook Natl Univ, Dept Vet Surg, Coll Vet Med, Daegu 41566, South Korea

来源：

VETERINARY SCIENCES | 2021年 / 8卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

feline; mesenchymal stem cells; exosomes; extracellular vesicles; anti-inflammation; EXTRACELLULAR VESICLES; DELIVERY VEHICLES; CYTOKINES; INTERLEUKIN-8; RANTES; RNA;

D O I：

10.3390/vetsci8090182

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Adipose tissue-derived mesenchymal stem cells (AD-MSCs) release extracellular vesicles such as exosomes, apoptotic bodies, and microparticles. In particular, exosomes are formed inside cells via multivesicular bodies (MVBs), thus their protein, DNA, and RNA content are similar to those of the parent cells. Exosome research is rapidly expanding, with an increase in the number of related publications observed in recent years; therefore, the function and application of MSC-derived exosomes could emerge as cell-free therapeutics. Exosomes have been isolated from feline AD-MSCs and feline fibroblast cell culture media using ultracentrifugation. Feline exosomes have been characterized by FACS, nanoparticle tracking analysis, and transmission electron microscopy imaging. Moreover, cytokine levels were detected by sandwich enzyme-linked immunosorbent assay in exosomes and LPS-induced THP-1 macrophages. The size of the isolated exosomes was that of a typical exosome, i.e., approximately 150 nm, and they expressed tetraspanins CD9 and CD81. The anti-inflammatory factor IL-10 was increased in feline AD-MSC-derived exosomes. However, pro-inflammatory factors such as IL-1 beta, IL-8, IL-2, RANTES, and IFN-gamma were significantly decreased in feline AD-MSC-derived exosomes. This was the first demonstration that feline AD-MSC-derived exosomes enhance the inflammatory suppressive effects and have potential for the treatment of immune diseases or as an inflammation-inhibition therapy.

引用

页数：12

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[11] Comparison of RNA isolation and library preparation methods for small RNA sequencing of canine biofluids [J].