Syzygium cumini Extract Decrease Adenosine Deaminase, 5′Nucleotidase Activities and Oxidative Damage in Platelets of Diabetic Patients

被引:27
作者
De Bona, Karine S. [1 ]
Belle, Luziane P. [2 ]
Sari, Marcel H. [1 ]
Thome, Gustavo [3 ]
Schetinger, Maria R. C. [3 ]
Morsch, Vera M. [3 ]
Boligon, Aline [1 ]
Athayde, Margareth L. [1 ]
Pigatto, Aline S. [4 ]
Moretto, Maria B. [1 ]
机构
[1] Univ Fed Santa Maria, Postgrad Program Pharmaceut Sci, Hlth Sci Ctr, BR-97105900 Santa Maria, RS, Brazil
[2] Univ Fed Santa Maria, Postgrad Program Pharmacol, BR-97105900 Santa Maria, RS, Brazil
[3] Univ Fed Santa Maria, Dept Chem, BR-97105900 Santa Maria, RS, Brazil
[4] Franciscan Univ Ctr, UNIFRA, Santa Maria, RS, Brazil
关键词
Adenosine deaminase; Diabetes mellitus; 5'Nucleotidase; Oxidative stress; Platelets; Syzygium cumini leaves extract; ALLIUM-SATIVUM L; 5'-NUCLEOTIDASE ACTIVITIES; LIPID-PEROXIDATION; EUGENIA-JAMBOLANA; ATP-DIPHOSPHOHYDROLASE; ANTIOXIDANT STATUS; CEREBRAL-CORTEX; ADIPOSE-TISSUE; RATS; MELLITUS;
D O I
10.1159/000322340
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Diabetes mellitus, a chronic metabolic disorder, has assumed epidemic proportions and its long-term complications can have devastating consequences. The oxidative stress in diabetes was greatly increased due to prolonged exposure to hyperglycemia and impairment of oxidant/antioxidant equilibrium. Syzygium cumini is being widely used to treat diabetes by the traditional practitioners over many centuries. Adenosine deaminase (ADA) and 5'-Nucleotidase (5'NT) are enzymes of purine nucleoside metabolism that play an important role in the regulation of adenosine (Ado) levels. In this study, we investigated the effect of Syzygium cumini aqueous leaves extract (ASc) on ADA and 5'NT activities and on parameters of oxidative stress under in vitro conditions, using platelets of patients with Type 2 diabetes mellitus. Platelet-Rich Plasma (PRP) was assayed by ADA, 5'NT, Catalase (CAT), Superoxide Dismutase (SOD) activities and Thiobarbituric acid reactive substances (TBARS) levels. We observed that ADA, 5'NT activities and TBARS levels were significantly higher when compared to the control group, and ASc (100 and 200 mu g/mL) prevented these effects. Our study demonstrates that ASc was able to remove oxidant species generated in diabetic conditions and modulates in the Ado levels. Then, ASc may promote a compensatory response in platelet function, improving the susceptibility-induced by the diabetes mellitus. Copyright (C) 2010 S. Karger AG, Basel
引用
收藏
页码:729 / 738
页数:10
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