Protein crystallization by combining laser irradiation and solution-stirring techniques

被引：10

作者：

Adachi, H

Niino, A

Murakami, S

Takano, K

Matsumura, H

Kinoshita, T

Warizaya, M

Inoue, T

Mori, Y

Sasaki, T

机构：

[1] Osaka Univ, Dept Elect Engn, Suita, Osaka 5650871, Japan

[2] Osaka Univ, SOSHO Project, Crystal Design Project, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Ctr Adv Sci & Innovat, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Dept Mat & Life Sci, Suita, Osaka 5650871, Japan

[5] Osaka Univ, Inst Sci & Ind Res, Osaka 5670047, Japan

[6] Osaka Univ, Dept Chem Mat, Suita, Osaka 5650871, Japan

[7] PRESTO, JST, Suita, Osaka 5650871, Japan

[8] Fujisawa Pharmaceut Co Ltd, Exploratory Res Labs, Tsukuba, Ibaraki 3002698, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS | 2005年 / 44卷 / 03期

关键词：

protein crystal; solution stirring; laser irradiation; nucleation; bovine adenosine deaminase;

D O I：

10.1143/JJAP.44.1365

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Bovine adenosine deaminase in the absence of an inhibitor (free-ADA) does not form crystals when using conventional crystallization methods. Using a solution-stirring technique, we recently succeeded in generating a small number of free-ADA crystals. In this paper, we demonstrate the combination of laser-irradiated growth and stirring (COLAS). This technique was found to be useful for controlling crystal nucleation and growth, which led to the production of a much larger number of high-quality free-ADA crystals.

引用

页码：1365 / 1366

页数：2

共 14 条

[1] Membrane protein crystallization using laser irradiation [J].

Adachi, H ;

Murakami, S ;

Niino, A ;

Matsumura, H ;

Takano, K ;

Inoue, T ;

Mori, Y ;

Yamaguchi, A ;

Sasaki, T .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS, 2004, 43 (10B) :L1376-L1378

[2] Generation of protein crystals using a solution-stirring technique [J].

Adachi, H ;

Niino, A ;

Matsumura, H ;

Takano, K ;

Kinoshita, T ;

Warizaya, M ;

Inoue, T ;

Mori, Y ;

Sasaki, T .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS, 2004, 43 (6B) :L762-L764

[3] Improving the quality of protein crystals using stirring crystallization [J].

Adachi, H ;

Matsumura, H ;

Niino, A ;

Takano, K ;

Kinoshita, T ;

Warizaya, M ;

Inoue, T ;

Mori, Y ;

Sasaki, T .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS, 2004, 43 (4B) :L522-L525

[4] Protein crystal growth with a two-liquid system and stirring solution [J].

Adachi, H ;

Takano, K ;

Matsumura, H ;

Inoue, T ;

Mori, Y ;

Sasaki, T .

JOURNAL OF SYNCHROTRON RADIATION, 2004, 11 (01) :121-124

[5] New practical technique for protein crystallization with floating and stirring methods [J].

Adachi, H ;

Matsumura, H ;

Takano, K ;

Niino, A ;

Inoue, T ;

Yoshimura, M ;

Mori, Y ;

Sasaki, T .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS, 2003, 42 (10A) :L1161-L1163

[6] Laser irradiated growth of protein crystal [J].

Adachi, H ;

Takano, K ;

Hosokawa, Y ;

Inoue, T ;

Mori, Y ;

Matsumura, H ;

Yoshimura, M ;

Tsunaka, Y ;

Morikawa, M ;

Kanaya, S ;

Masuhara, H ;

Kai, Y ;

Sasaki, T .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS, 2003, 42 (7B) :L798-L800

[7] Application of a stirring method to micro-scale and vapor diffusion protein crystallization [J].

Adachi, H ;

Takano, K ;

Yoshimura, M ;

Mori, Y ;

Sasaki, T .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS, 2003, 42 (3B) :L314-L315

[8] Promotion of large protein crystal growth with stirring solution [J].

Adachi, H ;

Takano, K ;

Yoshimura, M ;

Mori, Y ;

Sasaki, T .

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS, 2002, 41 (10A) :L1025-L1027

[9] Magnetic orientation as a tool to study the initial stage of crystallization of lysozyme [J].

Ataka, M ;

Katoh, E ;

Wakayama, NI .

JOURNAL OF CRYSTAL GROWTH, 1997, 173 (3-4) :592-596

[10] A test of macromolecular crystallization in microgravity: large well ordered insulin crystals [J].

Borgstahl, GEO ;

Vahedi-Faridi, A ;

Lovelace, J ;

Bellamy, HD ;

Snell, EH .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2001, 57 :1204-1207

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