Protective antigenic epitopes revealed by immunosignatures after three doses of inactivated SARS-CoV-2 vaccine

被引:4
作者
Peng, Mian [1 ,2 ,3 ]
Dou, Xiaowen [4 ]
Zhang, Xiuming [4 ]
Yan, Mingchen [5 ]
Xiong, Dan [4 ]
Jiang, Ruiwei [4 ]
Ou, Tong [4 ]
Tang, Aifa [6 ]
Yu, Xiqiu [7 ]
Zhu, Feiqi [8 ]
Li, Weiqin [1 ,2 ]
机构
[1] Southern Med Univ, Sch Clin Med 1, Guangzhou, Peoples R China
[2] Nanjing Univ, Affiliated Jinling Hosp, Dept Crit Care Med, Med Sch, Nanjing, Peoples R China
[3] Shenzhen Univ, Dept Crit Care Med, Affiliated Hosp 3, Shenzhen, Peoples R China
[4] Shenzhen Univ, Med Lab, Affiliated Hosp 3, Shenzhen, Peoples R China
[5] Shenzhen Digital Life Res Inst, Dept Artificial Intelligence & Bioinformat, Shenzhen, Peoples R China
[6] Shenzhen Univ, Sci & Educ Ctr, Affiliated Hosp 3, Shenzhen, Peoples R China
[7] Shenzhen Univ, Dept Gastroenterol, Affiliated Hosp 3, Shenzhen, Peoples R China
[8] Shenzhen Univ, Dept Neurol, Affiliated Hosp 3, Shenzhen, Peoples R China
来源
FRONTIERS IN IMMUNOLOGY | 2022年 / 13卷
关键词
protective antigenic epitopes; immunosignatures; three doses; inactivated; SARS-CoV-2; vaccine; B-CELLS; COVID-19; BINDING; PROTEIN;
D O I
10.3389/fimmu.2022.938378
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
BackgroundSARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) has infected millions of people around the world. Vaccination is a pillar in the strategy to control transmission of the SARS-CoV-2 spread. Immune responses to vaccination require elucidation. MethodsThe immune responses to vaccination with three doses of inactivated SARS-CoV-2 vaccine were followed in a cohort of 37 healthy adults (18-59 years old). Blood samples were collected at multiple time points and submitted to peptide array, machine learning modeling, and sequence alignment analyses, the results of which were used to generate vaccine-induced antibody-binding region (VIABR) immunosignatures (Registration number: ChiCTR2200058571). ResultsAntibody spectrum signals showed vaccination stimulated antibody production. Sequence alignment analyses revealed that a third vaccine dose generated a new highly represented VIABR near the A570D mutation, and the whole process of inoculation enhanced the VIABR near the N501Y mutation. In addition, the antigen conformational epitopes varied between short- and long-term samples. The amino acids with the highest scores in the short-term samples were distributed primarily in the receptor binding domain (RBD) and N-terminal domain regions of spike (S) protein, while in the long-term samples (12 weeks after the 2(nd) dose), some new conformational epitopes (CEs) were localized to crevices within the head of the S protein trimer. ConclusionProtective antigenic epitopes were revealed by immunosignatures after three doses of inactivated SARS-CoV-2 vaccine inoculation. A third dose results in a new top-10 VIABR near the A570D mutation site of S protein, and the whole process of inoculation enhanced the VIABR near the N501Y mutation, thus potentially providing protection from strains that have gained invasion and immune escape abilities through these mutation.
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页数:12
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