Hypoglycaemic and anti-ageing activities of green alga Ulva lactuca polysaccharide via gut microbiota in ageing-associated diabetic mice

被引:36
作者
Chen, Yihan [2 ]
Ouyang, Yuezhen [2 ]
Chen, Xuejin [4 ]
Chen, Ruoxin [2 ]
Ruan, Qiling [2 ]
Farag, Mohamed A. [5 ]
Chen, Xinhua [1 ,3 ]
Zhao, Chao [1 ,2 ,3 ,6 ]
机构
[1] Fujian Agr & Forestry Univ, Coll Marine Sci, Fuzhou 350002, Peoples R China
[2] Fujian Agr & Forestry Univ, Coll Food Sci, Fuzhou 350002, Peoples R China
[3] Fujian Agr & Forestry Univ, Inst Oceanol, Key Lab Marine Biotechnol Fujian Prov, Fuzhou 350002, Peoples R China
[4] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China
[5] Cairo Univ, Fac Pharm, Dept Pharmacognosy, Cairo 11562, Egypt
[6] 15 Shangxiadian Rd, Fuzhou 350002, Peoples R China
关键词
Ulva lactuca polysaccharide; Structure-activity relationship; Hypoglycaemic; Anti-ageing; Gut microbiota; IDENTIFICATION;
D O I
10.1016/j.ijbiomac.2022.05.109
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ageing-related type 2 diabetes is a significant public health problem. Particularly, the number of cases and fatality rates of ageing-associated diabetes increase with population ageing. This study aimed to investigate the structural characterisation of Ulva lactuca polysaccharide (ULP) and the hypoglycaemic effect on ageingassociated diabetic mice using gut microbiota variation. Sugar residuals analysis showed that the purified ULP (ULP-1) comprised beta-D-Xylp-(1 -> 3)-beta-D-Arap-(1 -> 6)-beta-D-Galp-(1 -> 6)-beta-D-Glcp linked to [->alpha-L-Rhap-(1 -> 4)-beta-DGlcpA ->]n and alpha-D-Manp-(1 -> 4)-alpha-L-Rhap(2SO3- )-(1 -> 2)-alpha-L-Rhap(4SO3- )-(1 -> 2)-alpha-L-Arap-(1 -> 2)-alpha-L-Rhap-(1 -> as its side chains at beta-D-Glcp. Moreover, ULP modulated the expression levels of p16Ink4a, MMP2, FoxO1, GLP-1/ GLP-1R, STAT3, and GLUT4 to improve the status of ageing and diabetes, which was concurrent with the increased abundance of Dubosiella, Enterococcus, Romboutsia, Bifidobacterium, Kurthia, Clostridium_sensu_stricto_1, Corynebacterium, Faecalibaculum, Aerococcus and Vibrio. Notably, Dubosiella, Romboutsia, Bifidobacterium, Turicibacter and Clostridium_sensu_stricto_1 could serve as important intermediates for delaying ageing and diabetes. Additionally, the ULP-1 structure is strongly binding interaction with the target protein through hydrogen bonding and Van der Waals force, especially for GLP-1 (-10.34 kcal/mol), p16Ink4a (-10.51 kcal/mol) and GLP1R (-8.57 kcal/mol). Moreover, the average length of the hydrogen bond was observed to be 2.36 MPa, which is smaller than that of the traditional hydrogen bond. Therefore, ULP has the potential to function as a nutraceutical to delay or prevent the development of ageing-related type 2 diabetes.
引用
收藏
页码:97 / 110
页数:14
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