Functional identification of MdSIZ1 as a SUMO E3 ligase in apple

被引：30

作者：

Zhang, Rui-Fen ^{[1
,2
,3
]}

Guo, Ying ^{[1
,2
,3
]}

Li, Yuan-Yuan ^{[1
,2
,3
]}

Zhou, Li-Jie ^{[1
,2
,3
]}

Hao, Yu-Jin ^{[1
,2
,3
]}

You, Chun-Xiang ^{[1
,2
,3
]}

机构：

[1] Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271000, Shandong, Peoples R China

[2] Shandong Agr Univ, MOA Key Lab Hort Crop Biol & Germplasm Innovat, Tai An 271000, Shandong, Peoples R China

[3] Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271000, Shandong, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2016年 / 198卷

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

Apple; MdSIZ1; SUMO E3 ligase; SUMOylation; Stress responses; Functional complementation; PROTEIN MODIFICATION; FRUIT COLORATION; ARABIDOPSIS; SIZ1; UBIQUITIN; SUMOYLATION; GENE; ACCUMULATION; ANTHOCYANIN; CONJUGATION;

D O I：

10.1016/j.jplph.2016.04.007

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

SUMOylation, the conjugation of target proteins with SUMO (small ubiquitin-related modifier), is a type of post-translational modification in eukaryotes and involves the sequential action of activation (El), conjugation (E2) and ligation (E3) enzymes. In Arabidopsis, the AtSIZ1 protein is a SUMO E3 ligase that promotes the conjugation of SUMO proteins to target substrates. Here, we isolated and identified a SUMO E3 ligase, MdSIZ1, in apple, which was similar to AtSIZ1. SUMOylation analysis showed that MdSIZ1 had SUMO E3 ligase activity in vitro and in vivo. SUMO conjugation was increased by high temperatures, low temperatures, and abscisic acid (ABA). The ectopic expression of MdSIZ1 in Arabidopsis sizl-2 mutant plants partially complemented the morphological mutant phenotype and enhanced the levels of SUMO conjugation. Taken together, these results suggest that MdSIZ1-mediated SUMO conjugation of target proteins is an important process that regulates the adaptation of apple plants to various environmental stresses. (C) 2016 Elsevier GmbH. All rights reserved.

引用

页码：69 / 80

页数：12