Correlation between the high expression levels of cancer-germline genes with clinical characteristics in esophageal squamous cell carcinoma

被引：14

作者：

Chen, Xinfeng ^{[1
,2
]}

Wang, Liping ^{[1
,2
]}

Yue, Dongli ^{[1
,2
]}

Liu, Jinyan ^{[1
,3
]}

Huang, Lan ^{[1
]}

Yang, Li ^{[1
]}

Cao, Ling ^{[1
,2
]}

Qin, Guohui ^{[1
,2
]}

Li, Anqi ^{[1
,2
]}

Wang, Dan ^{[1
]}

Wang, Meng ^{[1
,4
]}

Qi, Yu ^{[5
]}

Zhang, Bin ^{[6
]}

van der Bruggen, Pierre ^{[7
]}

Zhang, Yi ^{[1
,2
,3
,8
]}

机构：

[1] Zhengzhou Univ, Affiliated Hosp 1, Biotherapy Ctr, Zhengzhou 450052, Henan, Peoples R China

[2] Zhengzhou Univ, Affiliated Hosp 1, Dept Oncol, Zhengzhou, Henan, Peoples R China

[3] Zhengzhou Univ, Sch Life Sci, Zhengzhou, Henan, Peoples R China

[4] Zhengzhou Univ, Affiliated Hosp 1, Dept Gastroenterol, Zhengzhou, Henan, Peoples R China

[5] Zhengzhou Univ, Affiliated Hosp 1, Dept Cerebral Surg, Zhengzhou, Henan, Peoples R China

[6] Northwestern Univ, Sch Med, Dept Hematol Oncol, Chicago, IL USA

[7] Catholic Univ Louvain, de Duve Inst, Ludwig Inst Canc Res Brussels, Brussels, Belgium

[8] Engn Key Lab Cell Therapy Henan Prov, Zhengzhou, Henan, Peoples R China

来源：

HISTOLOGY AND HISTOPATHOLOGY | 2017年 / 32卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Esophageal squamous cell carcinoma (ESCC); Cancer-germline genes; Biomarker; Epithelial-mesenchymal transition; CYTOLYTIC T-LYMPHOCYTES; TESTIS ANTIGEN; TUMOR-ANTIGEN; MAGE-A; HEPATOCELLULAR-CARCINOMA; HETEROGENEOUS EXPRESSION; CANCER/TESTIS ANTIGENS; PROGNOSTIC RELEVANCE; LUNG-CANCER; MELANOMA;

D O I：

10.14670/HH-11-847

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Antigens encoded by cancer-germline genes are attractive targets for cancer immunotherapy. In this study, we aimed to evaluate the mRNA expression of cancer-germline genes, expression of the encoded proteins in patients with esophageal squamous cell carcinoma (ESCC) and their correlations with clinical characteristics. In addition, the effects of down regulation cancer-germline genes on ESCC cells were assessed in vitro. Our results showed that cancer-germline genes were frequently expressed in ESCC samples. The positive rates of in ESCC samples were: 87% of MAGE-A3, 60% of MAGE-A4, 65% of MAGE-C2, and 20% of NY-ESO-1 at mRNA level. MAGE-A3 expression was associated with age, lymph node metastasis and tumor stage (all P < 0.05), while MAGE-C2 expression was only associated with tumor stage (P < 0.05). Furthermore, the MAGE-A3 expressing patients had a poorer overall survival (P < 0.05). Multivariate analysis identified MAGE-A3 as an independent poor prognostic marker in ESCC. In vitro assay, ESCC cell lines treated with specific siRNAs to down-regulate MAGE-A3 and MAGE-C2 resulted in decreased colony-formation and migration ability (P < 0.05). Epithelial marker E-cadherin was up-regulated in siRNA-MAGE-A3/C2 cells compared to controls, whereas mesenchymal markers Vimentin, N-cadherin and Slug were downregulated (all P < 0.05), suggesting a role for MAGE-A3/C2 in ESCC metastasis through inducing epithelial-mesenchymal transition. The present study revealed that cancer germline genes and their encoded proteins were frequently expressed in ESCC tumor samples and were related to poor prognosis. Thus, cancer-germline genes may serve as useful biomarkers and potential targets for ESCC patients.

引用

页码：793 / 803

页数：11

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