Phosphodiesterase-1 Inhibitory Activity of Two Flavonoids Isolated from Pistacia integerrima J. L. Stewart Galls

被引：20

作者：

Rauf, Abdur ^{[1
]}

Saleem, Muhammad ^{[2
]}

Uddin, Ghias ^{[1
]}

Siddiqui, Bina S. ^{[2
]}

Khan, Haroon ^{[3
]}

Raza, Muslim ^{[1
]}

Hamid, Syeda Zehra ^{[1
]}

Khan, Ajmal ^{[2
]}

Maione, Francesco ^{[4
]}

Mascolo, Nicola ^{[4
]}

De Feo, Vincenzo ^{[5
]}

机构：

[1] Univ Peshawar, Inst Chem Sci, Peshawar 25120, Khyber Pakhtunk, Pakistan

[2] Univ Karachi, Int Ctr Chem & Biol Sci, HEJ Res Inst Chem, Karachi 75270, Pakistan

[3] Abdul Wali Khan Univ, Dept Pharm, Mardan 23200, Pakistan

[4] Univ Naples Federico II, Dept Pharm, I-80131 Naples, Italy

[5] Univ Salerno, Dept Pharm, I-84084 Salerno, Italy

来源：

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2015年 / 2015卷

关键词：

PULMONARY; ENVIRONMENT;

D O I：

10.1155/2015/506564

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Pistacia integerrima is one of twenty species among the genus Pistacia. Long horn-shaped galls that develop on this plant are harvested and used in Ayurveda and Indian traditional medicine to make "karkatshringi", a herbal medicine used for the treatment of asthma and different disorders of respiratory tract. However, until now, the molecular mechanisms of action of "karkatshringi" and its chemical characterization are partially known. This study deals with the isolation and characterization of the active constituents from the methanolic extract of P. integerrima galls and it was also oriented to evaluate in vitro and in silico their potential enzymatic inhibitory activity against phosphodiesterase-1 (PDE1), a well-known enzyme involved in airway smooth muscle activity and airway inflammation. Our results showed that the methanolic extract of P. integerrima galls and some of its active constituents [naringenin (1) and 3,5,7,4'-tetrahydroxy-flavanone (2)] are able in vitro to inhibit PDE1 activity (59.20 +/- 4.95%, 75.90 +/- 5.90%, and 65.25 +/- 5.25%, resp.) and demonstrate in silico an interesting interaction with this enzymatic site. Taken together, our results add new knowledge of chemical constituents responsible for the biological activity of P. integerrima and contextually legitimate the use of this plant in folk medicine.

引用

页数：6