Characterization and quantification of oxyntomodulin in human and rat plasma using high-resolution accurate mass LC-MS

被引：15

作者：

Cox, Jennifer M. ^{[1
]}

Berna, Michael J. ^{[1
]}

Jin, Zhaoyan ^{[1
]}

Cox, Amy L. ^{[1
]}

Sloop, Kyle W. ^{[1
]}

Gutierrez, Jesus A. ^{[1
]}

Ackermann, Bradley L. ^{[1
]}

机构：

[1] Eli Lilly & Co, Lilly Res Labs, Lilly Corp Ctr, Indianapolis, IN 46285 USA

来源：

BIOANALYSIS | 2016年 / 8卷 / 15期

关键词：

catabolites; high-resolution accurate mass MS; immunoaffinity enrichment; orbitrap; oxyntomodulin; quantification; validation; BIOACTIVE ENTEROGLUCAGON; ENERGY-EXPENDITURE; TRIPLE QUADRUPOLE; FOOD-INTAKE; Q-TOF; GLUCAGON; BIOANALYSIS; SENSITIVITY; MS/MS; QUANTITATION;

D O I：

10.4155/bio-2016-0012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: A thorough understanding of the biological role of oxyntomodulin (OXM) has been limited by the availability of sensitive and specific analytical tools for reliable in vivo characterization. Here, we utilized immunoaffinity capture coupled with high- resolution accurate mass LC- MS detection to quantify OXM and its primary catabolites. Results: Quantification of intact OXM 1-37 in human and rat plasma occurred in pre- and post-prandial samples. Profiles for the major catabolites were observed allowing kinetic differences to be assessed between species. Conclusion: A validated assay in human and rat plasma was obtained for OXM 1-37 and its catabolites, 3-37 and 4-37. The value of full scan high- resolution accurate mass detection without selected reaction monitoring for low- abundance peptide quantification was also demonstrated.

引用

页码：1579 / 1595

页数：17

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