A cross-sectional analysis of the effects of various centrifugation speeds and inclusion of the buffy coat in platelet-rich plasma preparation

被引:5
作者
Muthuprabakaran, K. [1 ]
Pai, Varadraj Vasant [1 ]
Ahmad, Suhail [1 ]
Shukla, Pankaj [1 ]
机构
[1] Goa Med Coll, Dept Dermatol, Bambolim, Goa, India
关键词
Buffy coat; centrifugation speed; platelet count; platelet-rich plasma; ACNE SCARS; ULCERS; EFFICACY; THERAPY; STORAGE; BLIND;
D O I
10.25259/IJDVL_1050_20
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Introduction: Platelet-rich plasma is an autologous blood preparation which is used in various medical specialties because of its regenerative properties. There is a wide variation in platelet-rich plasma preparation protocols and attaining the ideal platelet yield (>1 million platelets/mu L) in a clinic setting can be challenging. We aimed at analyzing the centrifuge spin rates at which to attain an ideal platelet-rich plasma yield and also to study the effect of inclusion of the buffy coat after the first spin on the final platelet concentration in platelet-rich plasma. Methods: Seventy-five whole blood samples were obtained and divided into two groups - (1) leukocyte-rich platelet-rich plasma group and (2) leukocyte-poor platelet-rich plasma group. Samples in both groups were centrifuged using the dual spin method, at one of three centrifugation speed combinations (initial "soft" spin and second "hard" spin speeds, respectively): (1) 100 g/400 g, (2) 350 g/1350 g and (3) 900 g/1800 g. Platelet, red blood cell (RBC) and white blood cell (WBC) counts in both groups were compared. Results: The 100 g/400 g spin gave a high platelet yield (increase of 395.4 +/- 111.1%) in the leukocyte-poor-platelet-rich plasma group, while in the leukocyte-rich platelet-rich plasma group both 100 g/400 g and 350 g/1350 g spins resulted in significantly higher yields with an increase of 691.5 +/- 316.3% and 738.6 +/- 193.3%, respectively. Limitations: The study was limited by a smaller sample size in the pure platelet-rich plasma (leukocyte-poor platelet-rich plasma) group. Conclusion: Ideal platelet yields can be achieved with both the 100 g/400 g as well as the 350 g/1350 g spins using the buffy coat inclusion method while the 100 g/400 g spin for "pure" platelet-rich plasma accomplishes a near-ideal platelet count with significantly reduced contamination with other cells.
引用
收藏
页码:792 / 799
页数:8
相关论文
共 50 条
[41]   Comparison of the effects of platelet-rich plasma and corticosteroid injection in rotator cuff disease treatment: a systematic review and meta-analysis [J].
Peng, Yundong ;
Li, Fengxing ;
Ding, Yue ;
Sun, Xianghong ;
Wang, Guanglan ;
Jia, Shaohui ;
Zheng, Cheng .
JOURNAL OF SHOULDER AND ELBOW SURGERY, 2023, 32 (06) :1303-1313
[42]   Relative Effects of Minoxidil 5%, Platelet-Rich Plasma, and Microneedling in Pattern Hair Loss: A Systematic Review and Network Meta-Analysis [J].
Gupta, Aditya K. ;
Wang, Tong ;
Bamimore, Mary A. ;
Ravi, Shruthi Polla ;
Talukder, Mesbah .
SKIN APPENDAGE DISORDERS, 2023, 9 (06) :397-406
[43]   The effects of platelet-rich plasma injection in knee and hip osteoarthritis: a meta-analysis of randomized controlled trials [J].
Yujie Dong ;
Butian Zhang ;
Qi Yang ;
Jiajing Zhu ;
Xiaojie Sun .
Clinical Rheumatology, 2021, 40 :263-277
[44]   Effects of Platelet-rich Plasma on Ocular Surface in Patients with Dry Eye Syndrome: Clinico-experimental Analysis [J].
Jung, Jae Uk ;
Lee, Sang Hee ;
Kim, Hong Kyun .
JOURNAL OF THE KOREAN OPHTHALMOLOGICAL SOCIETY, 2019, 60 (12) :1169-1175
[45]   Effects and safety of the combination of platelet-rich plasma (PRP) and hyaluronic acid (HA) in the treatment of knee osteoarthritis: a systematic review and meta-analysis [J].
Zhao Jinlong ;
Huang Hetao ;
Liang Guihong ;
Zeng Ling-feng ;
Yang Weiyi ;
Liu Jun .
BMC MUSCULOSKELETAL DISORDERS, 2020, 21 (01)
[46]   Effects of platelet-rich plasma in association with bone grafts in maxillary sinus augmentation: a systematic review and meta-analysis [J].
Lemos, C. A. A. ;
Mello, C. C. ;
dos Santos, D. M. ;
Verri, F. R. ;
Goiato, M. C. ;
Pellizzer, E. P. .
INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 2016, 45 (04) :517-525
[47]   Preparation Methods and Clinical Outcomes of Platelet-Rich Plasma for Intra-articular Hip Disorders: A Systematic Review and Meta-analysis of Randomized Clinical Trials [J].
Garcia, Flavio Luis ;
Williams, Brady T. ;
Polce, Evan M. ;
Heller, Daniel B. ;
Aman, Zachary S. ;
Nwachukwu, Benedict U. ;
Nho, Shane J. ;
Chahla, Jorge .
ORTHOPAEDIC JOURNAL OF SPORTS MEDICINE, 2020, 8 (10)
[48]   Clinical therapeutic effects of platelet-rich plasma in patients with burn wound healing A protocol for systematic review and meta-analysis [J].
Li, Jie ;
Hu, Wang-Ping ;
Zhong, Guo .
MEDICINE, 2021, 100 (31) :E26404
[49]   Effects of Platelet-Rich Plasma on Clinical Outcomes After Anterior Cruciate Ligament Reconstruction: A Systematic Review and Meta-analysis [J].
Zhu, Ting ;
Zhou, Jingbin ;
Hwang, Jooyeon ;
Xu, Xin .
ORTHOPAEDIC JOURNAL OF SPORTS MEDICINE, 2022, 10 (01)
[50]   Clinical Effects and Safety Outcomes of Platelet-Rich Plasma Therapy in Patients with Vasculogenic Erectile Dysfunction: A Systematic Review and Meta-Analysis [J].
Falcone, Marco ;
Preto, Mirko ;
Keskin, Hakan ;
Vazquez, Jesus Fernando Solorzano ;
Banthia, Ravi ;
Mahendran, Tara ;
Deger, Muslim Dogan ;
Vinod, K. V. ;
Putra, Rosadi ;
Sethupathy, Tamilselvi ;
Htiyar, Alperen Halil, I ;
Basiru, Afisu ;
Harraz, Ahmed M. ;
Shah, Rupin ;
Agarwal, Ashok .
WORLD JOURNAL OF MENS HEALTH, 2025,