Induction of apo'ptosis in the lung tissue from rats exposed to cigarette smoke involves p38/JNK MAPK pathway

被引:72
作者
Kuo, WH
Chen, JH
Lin, HH
Chen, BC
Hsu, JD
Wang, CJ
机构
[1] Chung Shan Med Univ, Inst Biochem & Biotechnol, Taichung 402, Taiwan
[2] Armed Forces Taichung Gen Hosp, Div Gastroenterol, Dept Internal Med, Taichung, Taiwan
[3] Chung Shan Med Univ Hosp, Dept Pathol, Taichung, Taiwan
关键词
apoptosis; cigarette smoke; lung pathogenesis; MAP kinase; caspase cascade;
D O I
10.1016/j.cbi.2005.04.008
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Smoking is a major cause of human lung cancer. Past studies suggest that apoptosis might influence the malignant phenotype, but little is known about the association between apoptosis and cigarette smoke (CS)-induced lung pathogenesis. Using an in situ cell death detection kit (TA300), the association of CS with apoptosis was determined in a concentration-dependent manner. Furthermore, the expression of related proteins were investigated in the terminal bronchiole areas of the lung tissue from rats exposed to CS. Results showed that the expression of phosphotyrosine proteins was increased significantly in lung tissue of rats exposed to CS from 5 to 15 cigarettes. Using Western blotting and immunoprecipitation assay, Fas, a death receptor, was proved just be one of these phosphotyrosine proteins. CS triggered activation of MAP kinase (p38/JNK or ERK2) pathway, which led to Jun or p53 phosphorylation and FasL induction links Fas phosphorylation. Further, smoke treatment produced an increase in the level of proapoptotic proteins (Bax, t-Bid, cytochrome c and caspase-3), but a decline in Bcl-2, procaspase-8 and procaspase-9 proteins. Thus, CS-induced apoptosis may result from two main mechanisms, one is the activation of p38/JNK-Jun-FasL signaling, and the other is stimulated by the stabilization of p53, increase in the ratio of Bax/Bcl-2, release of cytochrome c; thus, leading to activation of caspase cascade. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:31 / 42
页数:12
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