Apoptosis in procyclic Trypanosoma brucei rhodesiense in vitro

被引:0
|
作者
Welburn, SC
Dale, C
Ellis, D
Beecroft, R
Pearson, TW
机构
[1] UNIV LONDON LONDON SCH HYG & TROP MED,LONDON WC1E 7HT,ENGLAND
[2] UNIV VICTORIA,DEPT BIOCHEM & MICROBIOL,VICTORIA,BC V8W 3PX,CANADA
来源
CELL DEATH AND DIFFERENTIATION | 1996年 / 3卷 / 02期
关键词
apoptosis; Trypanosoma brucei rhodesiense; cell death; lectin;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Apoptosis is a phenomenon previously associated exclusively with metazoan organisms,We show here that procyclic insect form Trypanosoma brucei rhodesiense, a protozoan parasite, when treated in vitro with concanavalin A displayed several features normally associated with apoptosis in metazoan cells, Lectin treatment induced cleavage of nuclear DNA into oligonucleosomal fragments, suggesting activation of an endogenous nuclease in the parasite, Treated trypanosomes, although agglutinated and non-motile, exhibited fluorescence after treatment with the vital stain fluorescein diacetate and retained H-3-uridine indicating that their cell membranes remained intact during the period of DNA fragmentation. Electron micrographs showed characteristic morphology of cells undergoing apoptosis, including surface membrane vesiculation and migration of chromatin to the periphery of the nuclear membrane while mitochondria remained intact. These results suggest that treatment with concanavalin A triggers a cell death mechanism in T.b. rhodesiense similar to the process of apoptosis described in metazoa.
引用
收藏
页码:229 / 236
页数:8
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