Polystyrene shaping effect on the enriched bacterial community from the plastic-eating Alphitobius diaperinus (Insecta: Coleoptera)

被引:13
作者
Cucini, Claudio [1 ]
Funari, Rebecca [1 ]
Mercati, David [1 ]
Nardi, Francesco [1 ,2 ]
Carapelli, Antonio [1 ,2 ]
Marri, Laura [1 ,2 ]
机构
[1] Univ Siena, Dept Life Sci, I-53100 Siena, Italy
[2] Univ Naples Federico II, BAT Ctr, Interuniv Ctr Studies Bioinspired Agroenvironm Te, I-80138 Naples, Italy
关键词
Larval microbiome; Polystyrene; Enrichment culture; 16S rRNA; Alphitobius diaperinus; BIODEGRADATION; MINERALIZATION;
D O I
10.1007/s13199-022-00847-y
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Plastic pollution has become a serious issue of global concern, and biodegradation of plastic wastes is representing one attractive environment-friendly alternative to traditional disposal paths. It is known that insects are involved in the plastic polymer degradation process, with reported evidence of tenebrionid beetle larvae capable to degrade polystyrene (PS), one of the most used plastics worldwide. Recently, a ribosomal RNA based survey on the insect gut microbiota of the lesser mealworm Alphitobius diaperinus has revealed differentially abundant microbial taxa between PS-fed larvae and control group. Following these findings, an enrichment bacterial culture was set up in liquid carbon-free basal medium with PS film as sole carbon source using PS-fed larvae of A. diaperinus as inoculum. After two-months the culture was analysed both by molecular and culture-based methods. Isolated bacteria which had become prevalent under the selective enrichment conditions resulted ascribable to three taxonomic groups: Klebsiella, Pseudomonas, and Stenothrophomonas. The predominance of these groups in PS-fed larvae was confirmed by using bacterial 16S rRNA gene amplicon sequencing, and it was consistent with the results of previous reports. Isolated bacteria were able to attach to PS surfaces and SEM observations showed the presence of thin fibrillar structures connecting the bacterial cells to the abiotic surface.
引用
收藏
页码:305 / 313
页数:9
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