Microsatellite loci for the field cricket, Gryllus bimaculatus and their cross-utility in other species of Orthoptera
被引:18
作者:
Dawson, DA
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机构:
Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, EnglandUniv Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England
Dawson, DA
[1
]
Bretman, AJ
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机构:Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England
Bretman, AJ
Tregenza, T
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机构:Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England
Tregenza, T
机构:
[1] Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England
[2] Univ Leeds, Sch Biol, Leeds LS2 9JT, W Yorkshire, England
来源:
MOLECULAR ECOLOGY NOTES
|
2003年
/
3卷
/
02期
关键词:
cross-species amplification;
field cricket;
Gryllidae;
Gryllus bimaculatus;
microsatellite;
Orthoptera;
D O I:
10.1046/j.1471-8286.2003.t01-1-00407.x
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
We have isolated 16 microsatellite loci in the field cricket, Gryllus bimaculatus. Nine loci were found to be polymorphic in G. bimaculatus and the number of alleles varied from seven to 14. All 16 loci were tested for amplification in nine other species. In the five species tested belonging to the same subfamily (Gryllinae), a minimum of nine loci amplified. These loci will be used to determine paternity as part of a study to investigate the genetic benefits of polyandry.