AaWRKY17, a positive regulator of artemisinin biosynthesis, is involved in resistance to Pseudomonas syringae in Artemisia annua

被引：33

作者：

Chen, Tiantian ^{[1
]}

Li, Yongpeng ^{[1
]}

Xie, Lihui ^{[1
]}

Hao, Xiaolong ^{[2
]}

Liu, Hang ^{[1
]}

Qin, Wei ^{[1
]}

Wang, Chen ^{[1
]}

Yan, Xin ^{[1
]}

Wu-Zhang, Kuanyu ^{[1
]}

Yao, Xinghao ^{[1
]}

Peng, Bowen ^{[1
]}

Zhang, Yaojie ^{[1
]}

Fu, Xueqing ^{[1
]}

Li, Ling ^{[1
]}

Tang, Kexuan ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Agr & Biol,Joint Int Res Lab Metab & Dev Sci, Fudan SJTU Nottingham Plant Biotechnol R&D Ctr, Plant Biotechnol Res Ctr,Frontiers Sci Ctr Transf, Shanghai 200240, Peoples R China

[2] Zhejiang Chinese Med Univ, Coll Pharm, Lab Med Plant Biotechnol, Hangzhou 310053, Peoples R China

来源：

HORTICULTURE RESEARCH | 2021年 / 8卷 / 01期

基金：

国家重点研发计划; 比尔及梅琳达.盖茨基金会;

关键词：

WRKY TRANSCRIPTION FACTORS; BOTRYTIS-CINEREA; GENE; DEFENSE; OVEREXPRESSION; RESPONSES; ACID; DRUG;

D O I：

10.1038/s41438-021-00652-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Artemisia annua, a traditional Chinese medicinal plant, remains the only plant source for artemisinin production, yet few genes have been identified to be involved in both the response to biotic stresses, such as pathogens, and artemisinin biosynthesis. Here, we isolated and identified the WRKY transcription factor (TF) AaWRKY17, which could significantly increase the artemisinin content and resistance to Pseudomonas syringae in A. annua. Yeast one-hybrid (Y1H), dual-luciferase (dual-LUC), and electrophoretic mobility shift assay (EMSA) results showed that AaWRKY17 directly bound to the W-box motifs in the promoter region of the artemisinin biosynthetic pathway gene amorpha-4,11-diene synthase (ADS) and promoted its expression. Real-time quantitative PCR (RT-qPCR) analysis revealed that the transcript levels of two defense marker genes, Pathogenesis-Related 5 (PR5) and NDR1/HIN1-LIKE 10 (NHL10), were greatly increased in AaWRKY17-overexpressing transgenic A. annua plants. Additionally, overexpression of AaWRKY17 in A. annua resulted in decreased susceptibility to P. syringae. These results indicated that AaWRKY17 acted as a positive regulator in response to P. syringae infection. Together, our findings demonstrated that the novel WRKY transcription factor AaWRKY17 could potentially be used in transgenic breeding to improve the content of artemisinin and pathogen tolerance in A. annua.

引用

页数：11

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