Single-molecule fluorescence resonance energy transfer techniques on rotary ATP synthases

被引：24

作者：

Boersch, Michael ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Phys 3, D-70550 Stuttgart, Germany

来源：

BIOLOGICAL CHEMISTRY | 2011年 / 392卷 / 1-2期

关键词：

ABELtrap; anti-Brownian electrokinetic trap; FoF1-ATP synthase; fluorescence resonance energy transfer (FRET); single-molecule detection; subunit rotation; ESCHERICHIA-COLI; F0F1-ATP SYNTHASE; STEPWISE ROTATION; SUBUNIT ROTATION; BROWNIAN-MOTION; ELASTIC ENERGY; GAMMA-SUBUNIT; BINDING; RING; FRET;

D O I：

10.1515/BC.2011.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conformational changes of proteins can be monitored in real time by fluorescence resonance energy transfer (FRET). Two different fluorophores have to be attached to those protein domains which move during function. Distance fluctuations between the fluorophores are measured by relative fluorescence intensity changes or fluorescence lifetime changes. The rotary mechanics of the two motors of FoF1-ATP synthase have been studied in vitro by single-molecule FRET. The results are summarized and perspectives for other transport ATPases are discussed.

引用

页码：135 / 142

页数：8

共 49 条

[1] STRUCTURE AT 2.8-ANGSTROM RESOLUTION OF F1-ATPASE FROM BOVINE HEART-MITOCHONDRIA [J].

ABRAHAMS, JP ;

LESLIE, AGW ;

LUTTER, R ;

WALKER, JE .

NATURE, 1994, 370 (6491) :621-628

[2] Constant c10 Ring Stoichiometry in the Escherichia coli ATP Synthase Analyzed by Cross-Linking [J].

Ballhausen, Britta ;

Altendorf, Karlheinz ;

Deckers-Hebestreit, Gabriele .

JOURNAL OF BACTERIOLOGY, 2009, 191 (07) :2400-2404

[3] Stepwise rotation of the γ-subunit of EFoF1-ATP synthase during ATP synthesis:: a single-molecule FRET approach [J].

Börsch, M ;

Diez, M ;

Zimmermann, B ;

Trost, M ;

Steigmiller, S ;

Gräber, P .

MANIPULATION AND ANALYSIS OF BIOMOLECULES, CELLS AND TISSUES, 2003, 4962 :11-21

[4] Stepwise rotation of the γ-subunit of EF0F1-ATP synthase observed by intramolecular single-molecule fluorescence resonance energy transfer [J].

Börsch, M ;

Diez, M ;

Zimmermann, B ;

Reuter, R ;

Gräber, P .

FEBS LETTERS, 2002, 527 (1-3) :147-152

[5] THE BINDING CHANGE MECHANISM FOR ATP SYNTHASE - SOME PROBABILITIES AND POSSIBILITIES [J].

BOYER, PD .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1140 (03) :215-250

[6] Transient accumulation of elastic energy in proton translocating ATP synthase [J].

Cherepanov, DA ;

Mulkidjanian, AY ;

Junge, W .

FEBS LETTERS, 1999, 449 (01) :1-6

[7] Controlling Brownian motion of single protein molecules and single fluorophores in aqueous buffer [J].

Cohen, Adam E. ;

Moerner, W. E. .

OPTICS EXPRESS, 2008, 16 (10) :6941-6956

[8] Suppressing Brownian motion of individual biomolecules in solution [J].

Cohen, AE ;

Moerner, WE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (12) :4362-4365

[9] The Anti-Brownian ELectrophoretic trap (ABEL trap): Fabrication and software [J].

Cohen, AE .

Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, 2005, 5699 :296-305

[10] Proton-powered subunit rotation in single membrane-bound F0F1-ATP synthase [J].

Diez, M ;

Zimmermann, B ;

Börsch, M ;

König, M ;

Schweinberger, E ;

Steigmiller, S ;

Reuter, R ;

Felekyan, S ;

Kudryavtsev, V ;

Seidel, CAM ;

Gräber, P .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (02) :135-141

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