Cell Surface Display of Poly(3-hydroxybutyrate) Depolymerase and its Application

被引：3

作者：

Lee, Seung Hwan ^{[1
]}

Lee, Sang Yup ^{[2
]}

机构：

[1] Chonnam Natl Univ, Dept Biotechnol & Bioengn, Gwangju 61186, South Korea

[2] Korea Adv Inst Sci & Technol, Inst BioCentury, Dept Chem & Biomol Engn BK21 Program, Metab & Biomol Engn Natl Res Lab, Daejeon 34141, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2020年 / 30卷 / 02期

关键词：

Cell surface display; depolymerase; immobilization; enantioselective biocatalyst; OprF; ESCHERICHIA-COLI; HETEROLOGOUS PROTEINS; ANCHORING MOTIF; BACTERIAL; LIPASE; OPRF;

D O I：

10.4014/jmb.2001.01042

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have expressed extracellular poly(3-hydroxybutyrate) (PHB) depolymerase of Ralstonia pickettii T1 on the Escherichia coli surface using Pseudomonas OprF protein as a fusion partner by C-terminal deletion-fusion strategy. Surface display of depolymerase was confirmed by flow cytometry, immunofluorescence microscopy and whole cell hydrolase activity. For the application, depolymerase was used as an immobilized catalyst of enantioselective hydrolysis reaction for the first time. After 48 h, (R)-methyl mandelate was completely hydrolyzed, and (S)-mandelic acid was produced with over 99% enantiomeric excess. Our findings suggest that surface displayed depolymerase on E. coli can be used as an enantioselective biocatalyst.

引用

页码：244 / 247

页数：4

共 22 条

[1] Biotechnological applications of phage and cell display [J].

Benhar, I .

BIOTECHNOLOGY ADVANCES, 2001, 19 (01) :1-33

[2] Cell-surface display of heterologous proteins: From high-throughput screening to environmental applications [J].

Chen, W ;

Georgiou, G .

BIOTECHNOLOGY AND BIOENGINEERING, 2002, 79 (05) :496-503

[3] Efficient biodegradation of highly crystallized polyethylene terephthalate through cell surface display of bacterial PETase [J].

Chen, Zhuozhi ;

Wang, Yanyan ;

Cheng, Yingying ;

Wang, Xue ;

Tong, Shanwei ;

Yang, Haitao ;

Wang, Zefang .

SCIENCE OF THE TOTAL ENVIRONMENT, 2020, 709

[4] Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines [J].

Georgiou, G ;

Stathopoulos, C ;

Daugherty, PS ;

Nayak, AR ;

Iverson, BL ;

Curtiss, R .

NATURE BIOTECHNOLOGY, 1997, 15 (01) :29-34

[5] Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface [J].

Hiraishi, Tomohiro ;

Yamashita, Koichi ;

Sakono, Masafumi ;

Nakanishi, Jun ;

Tan, Liu-Tzea ;

Sudesh, Kumar ;

Abe, Hideki ;

Maeda, Mizuo .

MACROMOLECULAR BIOSCIENCE, 2012, 12 (02) :218-224

[6] Microbial degradation of polyhydroxyalkanoates [J].

Jendrossek, D ;

Handrick, R .

ANNUAL REVIEW OF MICROBIOLOGY, 2002, 56 :403-432

[7] Poly(3-hydroxybutyrate)-depolymerase from Pseudomonas lemoignei:: Catalysis of esterifications in organic media [J].

Kumar, A ;

Gross, RA ;

Jendrossek, D .

JOURNAL OF ORGANIC CHEMISTRY, 2000, 65 (23) :7800-7806

[8] Cell surface display of lipase in Pseudomonas putida KT2442 using OprF as an anchoring motif and its biocatalytic applications [J].

Lee, SH ;

Lee, SY ;

Park, BC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (12) :8581-8586

[9] Display of lipase on the cell surface of Escherichia coli using OprF as an anchor and its application to enantioselective resolution in organic solvent [J].

Lee, SH ;

Choi, JI ;

Han, MJ ;

Choi, JH ;

Lee, SY .

BIOTECHNOLOGY AND BIOENGINEERING, 2005, 90 (02) :223-230

[10] Display of bacterial lipase on the Escherichia coli cell surface by using FadL as an anchoring motif and use of the enzyme in enantioselective biocatalysis [J].

Lee, SH ;

Choi, JI ;

Park, SJ ;

Lee, SY ;

Park, BC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (09) :5074-5080

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