Directional culture of petroleum hydrocarbon degrading bacteria for enhancing crude oil recovery

被引：36

作者：

Li, Hailan ^{[1
]}

Lai, Ruiqiu ^{[1
]}

Jin, Yulin ^{[2
]}

Fang, Xinxiang ^{[2
]}

Cui, Kai ^{[1
,2
]}

Sun, Shanshan ^{[1
]}

Gong, Yejing ^{[1
]}

Li, Haonan ^{[1
]}

Zhang, Zhongzhi ^{[1
]}

Zhang, Guangqing ^{[3
]}

Zhang, Zhiyong ^{[1
]}

机构：

[1] China Univ Petr, State Key Lab Heavy Oil Proc, Beijing 102249, Peoples R China

[2] PetroChina Kalamay Petrochem Co Ltd, Petrochem Res Inst, Karamay 834003, Peoples R China

[3] Univ Wollongong, Sch Mech Mat Mechatron & Biomed Engn, Wollongong, NSW 2522, Australia

来源：

JOURNAL OF HAZARDOUS MATERIALS | 2020年 / 390卷

基金：

中国国家自然科学基金;

关键词：

Microbial enhanced oil recovery; Bacillus; Arcobacter; Nutrients; Microbial community; POLYCYCLIC AROMATIC-HYDROCARBONS; PSEUDOMONAS-AERUGINOSA STRAINS; MICROBIAL COMMUNITY STRUCTURE; BACILLUS-SUBTILIS; HYDROTHERMAL LIQUEFACTION; BIODEGRADATION; BIOSURFACTANT; DEGRADATION; FIELD; MICROORGANISMS;

D O I：

10.1016/j.jhazmat.2020.122160

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

An oxygen-constrained system of crude oil reservoir environment was constructed to stimulate the growth of indigenous microbes, such as petroleum hydrocarbon-degrading bacteria. Addition of nitrogen and phosphorus sources was investigated for the growth of petroleum hydrocarbon-degrading bacteria. The results show that nitrates and phosphates stimulated the growth of the bacteria and promoted the biodegradation of crude oil as the sole carbon source in this process. The minimum surface tension was 29.63 mN/m when the amounts of the nitrogen (NaNO3: (NH4)(2)SO4 = 2:1) and phosphorus (KH2PO4: NaH2PO4 = 5:2) sources added were 0.8 wt% and 1.4 wt%, respectively. Furthermore, the dominant petroleum hydrocarbon-degrading bacteria were shifted from Arcobacter in production water to Pseudomonas after the first subculture and then to Bacillus after the sixth subculture. The heteroatom groups in the crude oil were biodegraded simultaneously with normal alkanes and alkyl cyclohexanes. Addition of the nutrients resulted in microbial growth, microbial community shift, and enhanced microbial degradation.

引用

页数：10

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