Human RNA helicase II/Gu gene: Genomic organization and promoter analysis

被引：6

作者：

Zhu, KC ^{[1
]}

Henning, D ^{[1
]}

Valdez, B ^{[1
]}

Busch, H ^{[1
]}

机构：

[1] Baylor Coll Med, Dept Pharmacol, Houston, TX 77030 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 281卷 / 04期

关键词：

RNA helicase II; Gu protein; genomic organization; promoter analysis;

D O I：

10.1006/bbrc.2001.4446

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human RNA helicase II/Gu (hRH II/Gu) protein unwinds double-stranded RNA, folds single-stranded RNA and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. The genomic organization and the regulation of expression of the hRH II/Gu gene were analyzed. The hRH II/Gu gene is over 29 kb in length, and includes 15 exons. All intron-egon junctions contain consensus GT-AG sequences. An 85 bp basic promoter was identified which lacks a functional TATA box. In LNCaP cells, the hRH II/Gu promoter was up to 50 times as active as pGL2-control vector which has an SV 40 early promoter. Mutation of a putative c-Myc/USF binding site in the region between -110 and -85 caused a decrease in the promoter activity by 2- to 3-fold. A negative regulatory region was also found between -324 and -264. Studies of transcriptional regulation of hRH II/Gu under different physiological and pathophysiological conditions may provide clues to its biological function. (C) 2001 Academic Press.

引用

页码：1006 / 1011

页数：6

共 29 条

[1] BRCA1 protein is linked to the RNA polymerase II holoenzyme complex via RNA helicase a [J].