Purification and characterization of a lectin from the mushroom Mycoleptodonoides aitchisonii

被引：30
|
作者：
Kawagishi, H [1 ]
Takagi, J [1 ]
Taira, T [1 ]
Murata, T [1 ]
Usui, T [1 ]
机构：
[1] Shizuoka Univ, Fac Agr, Dept Appl Biol Chem, Shizuoka 4228529, Japan
来源：
PHYTOCHEMISTRY | 2001年 / 56卷 / 01期
关键词：
Mycoleptodonoides aitchisonii; climacodontaceae; mushrooms; fruiting body; lectin; surface plasmon resonance;
D O I：
10.1016/S0031-9422(00)00351-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A lectin was isolated from the mushroom Mycoleptodonoides aitchisonii by means of affinity chromatography on bovine submaxillary mucin (BSM)-Toyopearl and gel filtration on Superose 12 HR10/30 using a FPLC system. This lectin is composed of four identical 16 kDa subunits and the molecular mass of the intact lectin was estimated to be 64 kDa by gel filtration. In a hemagglutination inhibition assay, it exhibited strong sugar-binding specificity towards asialo-BSM among the mono- or oligo-saccharides and glycoproteins tested. The binding specificity of the lectin was also examined by surface plasmon resonance analysis. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：53 / 58
页数：6
相关论文
共 50 条
  • [21] A study of mycelial growth and exopolysaccharide production from a submerged culture of Mycoleptodonoides aitchisonii in an air-lift bioreactor
    Choi, DuBok
    Lee, Jun Han
    Kim, Yun-Soo
    Na, Myung-Sun
    Choi, On-You
    Lee, Hee-Duck
    Lee, Myung Koo
    Cha, Wol-Suk
    KOREAN JOURNAL OF CHEMICAL ENGINEERING, 2011, 28 (06) : 1427 - 1432
  • [22] Genotoxicity and Single-Treatment Toxicity Evaluation of Mycoleptodonoides aitchisonii (Agaricomycetes) Water Extract
    Lee, Soon-Young
    Park, Dae-Hun
    Kim, Ji-Young
    Lee, Young-Ju
    Oh, Deuk-Sil
    Bang, Mi-Ae
    Seo, Joung-Wook
    INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS, 2017, 19 (08) : 717 - 726
  • [23] Extraction, purification and physicochemical properties of a novel lectin from Laetiporus sulphureus mushroom
    Wang, Yufeng
    Wu, Benyue
    Shao, Jiangjuan
    Jia, Jinxia
    Tian, Youqiu
    Shu, Xu
    Ren, Xiaojie
    Guan, Yue
    LWT-FOOD SCIENCE AND TECHNOLOGY, 2018, 91 : 151 - 159
  • [24] Enhancing effect of Mycoleptodonoides aitchisonii on synthesis of nerve growth factor and releasing dopamine in the rat brain
    Okuyama, S
    Terashima, T
    Kawamura, Y
    Yokogoshi, H
    NUTRITIONAL NEUROSCIENCE, 2004, 7 (01) : 41 - 47
  • [25] PURIFICATION AND CHARACTERIZATION OF A NOVEL LECTIN FROM GEOTRUPES STERCORARIUS
    Devi, P. Rama
    Kombiah, P.
    Sudhakar, G. R. Learnal
    Babu, C.
    INTERNATIONAL JOURNAL OF ADVANCED BIOTECHNOLOGY AND RESEARCH, 2014, 5 (02): : 157 - 162
  • [26] Purification and characterization of a mitogenic lectin from Penicillium duclauxii
    Singh, Ram Sarup
    Walia, Amandeep Kaur
    Kennedy, John F.
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2018, 116 : 426 - 433
  • [27] Purification and characterization of a lectin from the starfish Asterias amurensis
    Yoshitaka Imamichi
    Yoshihiro Yokoyama
    Fisheries Science, 2013, 79 : 1007 - 1013
  • [28] Purification and characterization of a lectin from the starfish Asterias amurensis
    Imamichi, Yoshitaka
    Yokoyama, Yoshihiro
    FISHERIES SCIENCE, 2013, 79 (06) : 1007 - 1013
  • [29] Purification and functional characterization of lectin from Chenopodium album
    Mahwish Javed
    Muhammad Bilal
    Bushra Tabassum
    Arif Malik
    Olawale Samuel Adeyinka
    Muhammad Tariq
    Idrees Ahmad Nasir
    Journal of Proteins and Proteomics, 2022, 13 (1) : 55 - 62
  • [30] Purification and characterization of a lectin from Crotalaria paulina seeds
    Pando, LA
    de Carvalho, DD
    Toyama, MH
    Di Ciero, L
    Novello, JC
    Pascholatti, SF
    Marangoni, R
    PROTEIN JOURNAL, 2004, 23 (07): : 437 - 444
12345