On-chip modulation of evanescent illumination and live-cell imaging with polymer waveguides

被引：26

作者：

Agnarsson, Bjorn ^{[1
]}

Jonsdottir, Asta B. ^{[2
]}

Arnfinnsdottir, Nina B. ^{[1
]}

Leosson, Kristjan ^{[1
]}

机构：

[1] Univ Iceland, Inst Sci, Dept Phys, IS-107 Reykjavik, Iceland

[2] Univ Iceland, Fac Med, Canc Res Lab, IS-101 Reykjavik, Iceland

来源：

OPTICS EXPRESS | 2011年 / 19卷 / 23期

关键词：

REFLECTION FLUORESCENCE MICROSCOPY; LIGHT; FABRICATION; PLATFORM;

D O I：

10.1364/OE.19.022929

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Imaging of live cells was carried out using evanescent-wave excitation on a polymer waveguide chip. Integrated waveguide-based interferometric light modulators were fabricated in order to demonstrate on-chip control of excitation light, e. g., for time-lapse fluorescence microscopy. When combined with a sensitive high-resolution imaging system, the integrated waveguide-excitation platform provides an ideal method of near-surface studies of live cells, where photobleaching and/or phototoxicity effects are of critical concern. (C) 2011 Optical Society of America

引用

页码：22929 / 22935

页数：7

共 29 条

[21]

Pattison DI, 2006, EXP SUPPL, V96, P131

[22] An integrated optical interferometric nanodevice based on silicon technology for biosensor applications [J].

Prieto, F ;

Sepúlveda, B ;

Calle, A ;

Llobera, A ;

Domínguez, C ;

Abad, A ;

Montoya, A ;

Lechuga, LM .

NANOTECHNOLOGY, 2003, 14 (08) :907-912

[23] Optical biosensors for cell adhesion [J].

Ramsden, Jeremy J. ;

Horvath, Robert .

JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION, 2009, 29 (3-4) :211-223

[24] Optimizing performance of plasmonic devices for photonic circuits [J].

Rosenzveig, Tiberiu ;

Hermannsson, Petur Gordon ;

Boltasseva, Alexandra ;

Leosson, Kristjan .

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING, 2010, 100 (02) :341-346

[25] Total internal reflection fluorescence microscopy: technical innovations and novel applications [J].

Schneckenburger, H .

CURRENT OPINION IN BIOTECHNOLOGY, 2005, 16 (01) :13-18

[26] Light microscopy techniques for live cell Imaging [J].

Stephens, DJ ;

Allan, VJ .

SCIENCE, 2003, 300 (5616) :82-86

[27] Electron beam lithography:: resolution limits and applications [J].

Vieu, C ;

Carcenac, F ;

Pépin, A ;

Chen, Y ;

Mejias, M ;

Lebib, A ;

Manin-Ferlazzo, L ;

Couraud, L ;

Launois, H .

APPLIED SURFACE SCIENCE, 2000, 164 :111-117

[28]

Zamir E, 2001, J CELL SCI, V114, P3583

[29] Thermo-optic coefficients of polymers for optical waveguide applications [J].

Zhang, Zhiyi ;

Zhao, Ping ;

Lin, Peng ;

Sun, Fengguo .

POLYMER, 2006, 47 (14) :4893-4896

← 1 2 3 →