On-chip modulation of evanescent illumination and live-cell imaging with polymer waveguides

被引：26

作者：

Agnarsson, Bjorn ^{[1
]}

Jonsdottir, Asta B. ^{[2
]}

Arnfinnsdottir, Nina B. ^{[1
]}

Leosson, Kristjan ^{[1
]}

机构：

[1] Univ Iceland, Inst Sci, Dept Phys, IS-107 Reykjavik, Iceland

[2] Univ Iceland, Fac Med, Canc Res Lab, IS-101 Reykjavik, Iceland

来源：

OPTICS EXPRESS | 2011年 / 19卷 / 23期

关键词：

REFLECTION FLUORESCENCE MICROSCOPY; LIGHT; FABRICATION; PLATFORM;

D O I：

10.1364/OE.19.022929

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Imaging of live cells was carried out using evanescent-wave excitation on a polymer waveguide chip. Integrated waveguide-based interferometric light modulators were fabricated in order to demonstrate on-chip control of excitation light, e. g., for time-lapse fluorescence microscopy. When combined with a sensitive high-resolution imaging system, the integrated waveguide-excitation platform provides an ideal method of near-surface studies of live cells, where photobleaching and/or phototoxicity effects are of critical concern. (C) 2011 Optical Society of America

引用

页码：22929 / 22935

页数：7

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