On-chip modulation of evanescent illumination and live-cell imaging with polymer waveguides

被引：26
作者：
Agnarsson, Bjorn [1 ]
Jonsdottir, Asta B. [2 ]
Arnfinnsdottir, Nina B. [1 ]
Leosson, Kristjan [1 ]
机构：
[1] Univ Iceland, Inst Sci, Dept Phys, IS-107 Reykjavik, Iceland
[2] Univ Iceland, Fac Med, Canc Res Lab, IS-101 Reykjavik, Iceland
来源：
OPTICS EXPRESS | 2011年 / 19卷 / 23期
关键词：
REFLECTION FLUORESCENCE MICROSCOPY; LIGHT; FABRICATION; PLATFORM;
D O I：
10.1364/OE.19.022929
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Imaging of live cells was carried out using evanescent-wave excitation on a polymer waveguide chip. Integrated waveguide-based interferometric light modulators were fabricated in order to demonstrate on-chip control of excitation light, e. g., for time-lapse fluorescence microscopy. When combined with a sensitive high-resolution imaging system, the integrated waveguide-excitation platform provides an ideal method of near-surface studies of live cells, where photobleaching and/or phototoxicity effects are of critical concern. (C) 2011 Optical Society of America
引用
收藏
页码：22929 / 22935
页数：7
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