Haploids and doubled haploids in Brassica spp. for genetic and genomic research

被引：71
作者：
Ferrie, Alison M. R. [1 ]
Moellers, Christian [2 ]
机构：
[1] CNR, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
[2] Univ Gottingen, Dept Nutzpflanzenwissensch, Abt Pflanzenzuchtung, D-37075 Gottingen, Germany
来源：
PLANT CELL TISSUE AND ORGAN CULTURE | 2011年 / 104卷 / 03期
关键词：
Doubled haploids; Breeding; Transformation; Mutagenesis; Genomics; MICROSPORE-DERIVED EMBRYOS; IN-VITRO SELECTION; MOLECULAR MARKERS; OIL CONTENT; NAPUS L; IMPROVED RESISTANCE; SUBSTITUTION LINES; LINKAGE ANALYSIS; ACID CONTENT; QTL ANALYSIS;
D O I：
10.1007/s11240-010-9831-4
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The availability of a highly efficient and reliable microspore culture protocol for many Brassica species makes this system useful for studying basic and applied research questions. Microspores and microspore-derived embryos are ideal targets for modification by mutagenesis and transformation. Regenerated doubled haploid plants are widely used in breeding programs and in genetic studies. Furthermore, the Brassica microspore culture system allows the identification of genomic regions and genes involved in the microspore embryogenic response, spontaneous diploidization and direct embryo to plant conversion. This review summarizes current achievements and discusses future perspectives.
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页码：375 / 386
页数：12
相关论文
共 88 条
  • [1] High frequency production of rapeseed transgenic plants via combination of microprojectile bombardment and secondary embryogenesis of microspore-derived embryos
    Abdollahi, M. R.
    Moieni, A.
    Mousavi, A.
    Salmanian, A. H.
    [J]. MOLECULAR BIOLOGY REPORTS, 2011, 38 (02) : 711 - 719
  • [2] Secondary embryogenesis and transient expression of the β-glucuronidase gene in hypocotyls of rapeseed microspore-derived embryos
    Abdollahi, M. R.
    Moieni, A.
    Salmanian, A. H.
    Mousavi, A.
    [J]. BIOLOGIA PLANTARUM, 2009, 53 (03) : 573 - 577
  • [3] An efficient method for transformation of pre-androgenic, isolated Brassica napus microspores involving microprojectile bombardment and Agrobacterium-mediated transformation
    Abdollahi, M. R.
    Corral-Martinez, P.
    Mousavi, A.
    Salmanian, A. H.
    Moieni, A.
    Segui-Simarro, J. M.
    [J]. ACTA PHYSIOLOGIAE PLANTARUM, 2009, 31 (06) : 1313 - 1317
  • [4] Characterisation of transgenic oilseed rape expressing pea lectin in anthers for improved resistance to pollen beetle
    Ahman, Inger M.
    Kazachkova, Nadiya I.
    Kamnert, Irene M.
    Hagberg, Per A.
    Dayteg, Christophe I.
    Eklund, G. Monica
    Meijer, L. Johan O.
    Ekbom, Barbara
    [J]. EUPHYTICA, 2006, 151 (03) : 321 - 330
  • [5] Mapping of loci affecting the cultural efficiency of microspore culture of Brassica rapa L-syn. campestris L-using DNA polymorphism
    Ajisaka, H
    Kuginuki, Y
    Shiratori, M
    Ishiguro, K
    Enomoto, S
    Hirai, M
    [J]. BREEDING SCIENCE, 1999, 49 (03) : 187 - 192
  • [6] Modification of glucosinolate and erucic acid contents in doubled haploid lines of Brassica carinata by UV treatment of isolated microspores
    Barro, F
    Fernández-Escobar, J
    De la Vega, M
    Martín, A
    [J]. EUPHYTICA, 2003, 129 (01) : 1 - 6
  • [7] Response of different genotypes of Brassica carinata to microspore culture
    Barro, F
    Martín, A
    [J]. PLANT BREEDING, 1999, 118 (01) : 79 - 81
  • [8] Doubled haploid lines of Brassica carinata with modified erucic acid content through mutagenesis by EMS treatment of isolated microspores
    Barro, F
    Fernández-Escobar, J
    De La Vega, M
    Martín, A
    [J]. PLANT BREEDING, 2001, 120 (03) : 262 - 264
  • [9] Reduction of saturated fats by mutagenesis and heat selection in Brassica napus L.
    Beaith, ME
    Fletcher, RS
    Kott, LS
    [J]. EUPHYTICA, 2005, 144 (1-2) : 1 - 9
  • [10] BEVERSDORF WD, 1987, IOWA STATE J RES, V61, P435
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