Functional Analysis of the Cyclopiazonic Acid Biosynthesis Gene Cluster in Aspergillus oryzae RIB 40

被引：10
|
作者：
Shinohara, Yasutomo [1 ]
Tokuoka, Masafumi [1 ]
Koyama, Yasuji [1 ]
机构：
[1] Noda Inst Sci Res, Noda, Chiba 2780037, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2011年 / 75卷 / 11期
关键词：
Aspergillus oryzae; cyclopiazonic acid; biosynthesis gene cluster; secondary metabolism; SECONDARY METABOLISM; FLAVUS; LAEA;
D O I：
10.1271/bbb.110467
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cyclopiazonic acid (CPA) nonproducing strain, Aspergillus oryzae RIB 40, does not biosynthesize cycloacetoacetyl-L-tryptophan (cAATrp) due to a truncation in the responsible PKS-NRPS gene. We found that RIB 40 converted cAATrp to 2-oxocyclopiazonic acid, the final product of CPA biosynthesis in A. oryzae. This indicates that the CPA biosynthesis gene cluster, except for the PKS-NRPS gene, is functional in RIB 40.
引用
收藏
页码：2249 / 2252
页数：4
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