Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage

被引:60
作者
Tohno, Masanori [1 ]
Kobayashi, Hisami
Nomura, Masaru [2 ]
Kitahara, Maki [3 ]
Ohkuma, Moriya [3 ]
Uegaki, Ryuichi
Cai, Yimin
机构
[1] Natl Inst Livestock & Grassland Sci, Funct Feed Res Team, Natl Agr & Food Res Org, Nasushiobara, Tochigi 3292793, Japan
[2] Natl Inst Livestock & Grassland Sci, Natl Agr & Food Res Org, Tsukuba, Ibaraki, Japan
[3] RIKEN BioResource Ctr, Wako, Saitama, Japan
基金
日本学术振兴会;
关键词
16S rDNA sequencing; Italian ryegrass; lactic acid bacteria; rapid PCR-based analysis; silage; CURVATUS SUBSP MELIBIOSUS; FORAGE CROPS; LACTOBACILLUS-CURVATUS; FERMENTATION CHARACTERISTICS; PCR; DIVERSITY; ALFALFA; IDENTIFICATION; POPULATIONS; SUCCESSION;
D O I
10.1111/j.1740-0929.2011.00923.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Twenty-three lactic acid bacteria (LAB) isolated from three cultivars (Akiaoba, Nagahahikari and Tachiwase) of Italian ryegrass (Lolium multiflorum Lam.) silage were precisely characterized by a combination of phenotypic tests, genotypic 16S ribosomal DNA sequencing and rapid PCR-based analyses, focusing on their useful phenotypes for silage preparation as inoculants. We successfully identified both at the species and subspecies levels: phenotypically novel Lactococcus lactis subsp. lactis, Lactobacillus brevis, Lactobacillus coryniformis subsp. torquens, Lactobacillus curvatus, Lactobacillus plantarum subsp. plantarum, Lactobacillus sakei subsp. carnosus, Leuconostoc mesenteroides subsp. dextranicum and Pediococcus parvulus. This is the first report to elucidate the presence of Lactobacillus coryniformis ssp. torquens and Leuconostoc mesenteroides subsp. dextranicum in Italian ryegrass silages. Physiological and biochemical tests revealed that phenotypic characteristics are different among the different strains of the same species and subspecies, and that the isolates show unique and diverse phenotypes related to fermentation factors, such as available carbohydrates, optimal growth pH and temperature. These results suggest that, for various well-preserved silage preparations, the isolates may be useful in producing novel inoculants corresponding to their optimally climatic and ecological niches.
引用
收藏
页码:111 / 120
页数:10
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