Interlaboratory proficiency test for detection of Paenibacillus larvae, the causative agent of American foulbrood

被引:0
作者
Schaefer, Marc Oliver [1 ,3 ]
von der Ohe, Werner [2 ]
Beims, Hannes [2 ]
Fischer, Susanne [3 ]
机构
[1] Bundesforschungsinst Tiergesundheit, Friedrich Loeffler Inst FLI, Natl Referenzlab Bienenkrankheiten, Greifswald, Germany
[2] Niedersachs Landesamt Verbraucherschutz & Lebensm, Inst Bienenkunde Celle, Celle, Germany
[3] Bundesforschungsinst Tiergesundheit, Friedrich Loeffler Inst FLI, Inst Infekt Med IMED, Greifswald, Germany
来源
BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT | 2020年 / 133卷
关键词
diagnostics; laboratory comparison test; Paenibacillus larvae; standardization; ETIOLOGIC AGENT; RECLASSIFICATION; VIRULENCE; GENOTYPES; HONEY;
D O I
10.2376/1439-0299-2020-20
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In autumn 2018, the German National Reference Laboratory for American foulbrood carried out a nationwide proficiency test with an included laboratory comparison test in cooperation with the LAVES - Institute for Apiculture, in which a total of 26 laboratories took part, including one from abroad. Identical sample panels, consisting of seven honey samples with a volume of at least 30 g each, were sent in coded form to all participants with the request that they be examined within a specified period of time using the methods prescribed for the proficiency test and the methods used in the routine of the laboratory. The results are shown and commented in the article. They confirm the good status of AFB diagnostics and the quality of the facilities in Germany, but also the need for harmonization and standardization of the materials used for diagnostics, especially the culture media.
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页数:7
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