Pyrosequencing-based barcodes for a dye-free multiplex bioassay

被引：10

作者：

Chen, Zhiyao ^{[1
]}

Fu, Xiaoying ^{[2
]}

Zhang, Xiaodan ^{[2
]}

Liu, Xiqun ^{[2
]}

Zou, Bingjie ^{[2
]}

Wu, Haiping ^{[2
]}

Song, Qinxin ^{[1
]}

Li, Jinheng ^{[1
]}

Kajiyama, Tomoharu ^{[3
]}

Kambara, Hideki ^{[3
]}

Zhou, Guohua ^{[1
,2
]}

机构：

[1] Nanjing Univ, Sch Med, Jinling Hosp, Dept Pharmacol, Nanjing 210002, Jiangsu, Peoples R China

[2] Huadong Res Inst Med & Biotech, Nanjing 210002, Jiangsu, Peoples R China

[3] Hitachi Ltd, Cent Res Lab, Kokubunji, Tokyo 1858601, Japan

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 18期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1039/c2cc17618a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel dye-free labeling method for a multiplex bioassay was proposed by using short sequence-based barcodes consisting of a reporter base and repeats of two stuffer bases; then, the barcodes were quantitatively decoded by a single pyrosequencing assay without any pre-separation.

引用

页码：2445 / 2447

页数：3

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