Antifibrinolytic Role of a Bee Venom Serine Protease Inhibitor That Acts as a Plasmin Inhibitor

被引：68
作者：
Choo, Young Moo [1 ]
Lee, Kwang Sik [1 ]
Yoon, Hyung Joo [2 ]
Qiu, Yuling [1 ]
Wan, Hu [1 ]
Sohn, Mi Ri [1 ]
Sohn, Hung Dae [1 ]
Jin, Byung Rae [1 ]
机构：
[1] Dong A Univ, Coll Nat Resources & Life Sci, Pusan, South Korea
[2] Natl Acad Agr Sci, Dept Agr Biol, Suwon, South Korea
来源：
PLOS ONE | 2012年 / 7卷 / 02期
关键词：
PSEUDONAJA-TEXTILIS; BLOOD-COAGULATION; APROTININ; EVOLUTION; PURIFICATION; HEMOSTASIS; ENZYME;
D O I：
10.1371/journal.pone.0032269
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Bee venom is a rich source of pharmacologically active substances. In this study, we identified a bumblebee (Bombus ignitus) venom Kunitz-type serine protease inhibitor (Bi-KTI) that acts as a plasmin inhibitor. Bi-KTI showed no detectable inhibitory effect on factor Xa, thrombin, or tissue plasminogen activator. In contrast, Bi-KTI strongly inhibited plasmin, indicating that it acts as an antifibrinolytic agent; however, this inhibitory ability was two-fold weaker than that of aprotinin. The fibrin(ogen)olytic activities of B. ignitus venom serine protease (Bi-VSP) and plasmin in the presence of Bi-KTI indicate that Bi-KTI targets plasmin more specifically than Bi-VSP. These findings demonstrate a novel mechanism by which bumblebee venom affects the hemostatic system through the antifibrinolytic activity of Bi-KTI and through Bi-VSP-mediated fibrin(ogen) olytic activities, raising interest in Bi-KTI and Bi-VSP as potential clinical agents.
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