Evaluation of pentaproline-based chiral stationary phase by LC

A pentaproline-based chiral stationary phase was prepared and the selectivity of the column was evaluated with 194 racemic compounds in three mobile phase modes: normal-phase mode, polar organic mode and reversed-phase mode. 94 racemates out of 194 were separated and the normal-phase mode proved to be the separation mode of broadest applicability. The column is stable in all common organic solvents and no degradation in column performance was observed in any mode even after more than 1,000 injections. A brief sample loading test was performed on the 250 mm x 4.6 mm column and 13.2 mg of alpha-methyl-9-anthracenemethanol was baseline separated. Retention behavior in the normal-phase mode and the effect of analyte structure on retention and enantioselectivity are discussed.