Sustained release of plasmid DNA from PLLA/POSS nanofibers for angiogenic therapy

被引：35

作者：

Li, Wenqiang ^{[1
,2
]}

Wu, Dongwei ^{[1
,2
]}

Zhu, Shanshan ^{[1
,2
]}

Liu, Zhibin ^{[1
,2
]}

Luo, Binghong ^{[1
,2
]}

Lu, Lu ^{[1
,2
]}

Zhou, Changren ^{[1
,3
]}

机构：

[1] Jinan Univ, Dept Mat Sci & Engn, Guangzhou 510632, Guangdong, Peoples R China

[2] Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China

[3] Jinan Univ, Guangzhou, Peoples R China

来源：

CHEMICAL ENGINEERING JOURNAL | 2019年 / 365卷

基金：

中国国家自然科学基金;

关键词：

Nanofibrous scaffold; Poly(lactic acid); Polyhedral oligomeric silsesquioxane; DNA plasmid; Sustained release; Angiogenesis; GROWTH-FACTORS; LASER PHOTOBIOMODULATION; MECHANICAL-PROPERTIES; 3RD-DEGREE BURNS; GENE DELIVERY; ELECTROSPUN; SCAFFOLD; VASCULARIZATION; CELLS; OSTEOGENESIS;

D O I：

10.1016/j.cej.2019.02.043

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Angiogenesis plays a critical role to provide a desired microenvironment during tissue repair and regeneration. Gene-activated scaffold is increasingly showing potential in tissue engineering and regenerative medicine. Herein, functional polyhedral oligomeric silsesquioxane (POSS) nanoparticles, known for the organic-inorganic structure and biocompatibility, were incorporated into poly(L-lactic acid) (PLLA) matrix and processed by electrospinning into nanofibers as a delivery vehicle of plasmid DNA encoding angiopoietin-1 (pAng). The introduction of functional POSS nanoparticles increased the strength and toughness of the matrix simultaneously, and formed the porous fiber structure. PAng released from the nanofibrous scaffold showed sustained delivery over 35 days and maintained high transfection efficiency. A full-thickness skin wound model was used to evaluate the pAng delivery efficacy and angiogenesis capability of the plasmid DNA-based polymeric scaffold in vivo. The results indicated that the pAng loaded PLLA/POSS scaffold effectively promoted angiogenesis and dermal wound healing.

引用

页码：270 / 281

页数：12

共 49 条

[1] Electrospun biphasic tubular scaffold with enhanced mechanical properties for vascular tissue engineering [J].