Transcriptional accuracy modeling suggests two-step proofreading by RNA polymerase

被引:10
|
作者
Mellenius, Harriet [1 ]
Ehrenberg, Mans [1 ]
机构
[1] Uppsala Univ, Dept Cell & Mol Biol, S-75237 Uppsala, Sweden
基金
瑞典研究理事会;
关键词
STRUCTURAL BASIS; TRIGGER LOOP; FIDELITY; ELONGATION; MECHANISM; DNA; RECOGNITION; MISMATCHES; CLEAVAGE; ERRORS;
D O I
10.1093/nar/gkx849
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We suggest a novel two-step proofreading mechanism with two sequential rounds of proofreading selection in mRNA transcription. It is based on the previous experimental observations that the proofreading RNA polymerase cleaves off transcript fragments of at least 2 nt and that transcript elongation after a nucleotide misincorporation is anomalously slow. Taking these results into account, we extend the description of the accuracy of template guided nucleotide selection beyond previous models of RNA polymerase-dependent DNA transcription. The model derives the accuracy of initial and proofreading base selection from experimentally estimated nearest-neighbor parameters. It is also used to estimate the small accuracy enhancement of polymerase revisiting of previous positions following transcript cleavage.
引用
收藏
页码:11582 / 11593
页数:12
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